Multi-omic strategies for transcriptome-wide prediction and association studies

BioRxiv : the Preprint Server for Biology
A. Bhattacharya, Michael I. Love


Traditional predictive models for transcriptome-wide association studies (TWAS) consider only single nucleotide polymorphisms (SNPs) local to genes of interest and perform parameter shrinkage with a regularization process. These approaches ignore the effect of distal-SNPs or possible effects underlying the SNP-gene association. Here, we outline multi-omic strategies for transcriptome imputation from germline genetics for testing gene-trait associations by prioritizing distal-SNPs to the gene of interest. In one extension, we identify mediating biomarkers (CpG sites, microRNAs, and transcription factors) highly associated with gene expression and train predictive models for these mediators using their local SNPs. Imputed values for mediators are then incorporated into the final model as fixed effects with local SNPs to the gene included as regularized effects. In the second extension, we assess distal-eSNPs (SNPs in eQTLs) for their mediation effect through mediators local to these distal-eSNPs. Highly mediated distal-eSNPs are then included in the eventual transcriptomic prediction model. We show considerable gains in percent variance explained of gene expression and TWAS power to detect gene-trait associations using simulation...Continue Reading

Related Concepts

Computer Software
Gene Polymorphism
Short Tandem Repeat
Electrophoresis, Capillary
Nucleic Acid Sequencing
High Throughput Analysis
Human Identification

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

© 2020 Meta ULC. All rights reserved