Human coelomic fluid investigation: A MS-based analytical approach to prenatal screening

Scientific Reports
Donatella AielloAnna Napoli

Abstract

Coelomic fluid (CF) is the earliest dynamic and complex fluid of the gestational sac. CF contains maternal cells and proteins produced by embryonic cells, tissues and excretions. The biochemical composition of CF is modified throughout the first trimester of pregnancy and its protein profile reflects both physiological/pathological changes affecting the embryo and mother. Identification of variations in the balance of proteins might indicate particular types of pathologies, or ascertain specific genetic disorders. A platform utilizing protein enrichment procedures coupled with shotgun identification and iTRAQ differentiation provided the identification and quantitation of 88 unique embryonic proteins. It is relevant to note that chromosome X protein CXorf23 was found suggesting the embryo sex. Foetal sex was determined by Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR) on coelomic cells, foetal tissues and maternal white blood cells, with a 100% concordance rate between iTRAQ-MS/MS and QF-PCR data. The functional associations among the identified proteins were investigated using STRING database. Open Targets Platform showed as significant the following therapeutic areas: nervous, respiratory, eye and head system dis...Continue Reading

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Citations

Mar 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Donatella AielloAnna Napoli
Mar 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Andrea TemperiniCarlo Siciliano
Mar 17, 2020·International Journal of Biological Macromolecules·Luca SalvatoreAlessandro Sannino
Apr 28, 2021·Journal of Proteome Research·Donatella AielloAnna Napoli

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Methods Mentioned

BETA
electrophoresis
immunodepletion
Polymerase Chain Reaction
PCR
reversed phase chromatography

Software Mentioned

OTP
INTERPRO
Open Targets Platform ( OTP )
Gene Mapper
Protein Pilot

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