Human colon carcinoma cells use multiple receptors to adhere to laminin: involvement of alpha 6 beta 4 and alpha 2 beta 1 integrins

Cell Regulation
M M LotzA M Mercurio

Abstract

In this study, we used clone A, a human colon carcinoma cell line, to characterize those integrins that mediate colon carcinoma adhesion to laminin. Monoclonal antibodies specific for the human beta 1 subunit inhibited clone A adhesion to laminin. They also precipitated a complex of surface proteins that exhibited an electrophoretic behavior characteristic of alpha 2 beta 1 and alpha 3 beta 1. A monoclonal antibody specific for alpha 2 (PIH5) blocked clone A adhesion to laminin, as well as to collagen I. An alpha 3-specific antibody (P1B5) had no effect on clone A adhesion to laminin, even though it can block the adhesion of other cell types to laminin. Thus, the alpha 2 beta 1 integrin can function as both a laminin and collagen I receptor on clone A cells. Although these cells express alpha 3 beta 1, an established laminin receptor, they do not appear to use it to mediate laminin adhesion. In addition, the monoclonal antibody GoH3, which recognizes the alpha 6 integrin subunit, also inhibited carcinoma adhesion to laminin but not to fibronectin or collagen I. This antibody precipitated the alpha 6 subunit in association with the beta 4 subunit. There was no evidence of alpha 6 beta 1 association on these cells. In summary, th...Continue Reading

References

Aug 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A SonnenbergJ Hilgers
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U M WewerR Albrechtsen
Mar 1, 1988·The Journal of Cell Biology·K R GehlsenE Ruoslahti
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M J Elices, M E Hemler
Jul 1, 1989·The Journal of Cell Biology·R O HynesG H Yee
May 31, 1988·Biochemical and Biophysical Research Communications·S A SantoroV L Woods
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Pignatelli, W F Bodmer
Feb 1, 1986·The Journal of Cell Biology·S K AkiyamaK M Yamada
Dec 1, 1988·Nature·A SonnenbergF Hogervorst
Jan 1, 1987·Journal of Cell Science. Supplement·C A Buck, A F Horwitz

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Citations

Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A M Mercurio, L M Shaw
Jan 1, 1994·Breast Cancer Research and Treatment·M Maemura, R B Dickson
Jan 1, 1994·In Vitro Cellular & Developmental Biology. Animal·M J HoracekL Terracio
Aug 17, 2004·International Journal of Colorectal Disease·Marc JansenVolker Schumpelick
Oct 29, 1993·European Journal of Obstetrics, Gynecology, and Reproductive Biology·P BischofA Campana
Jan 1, 1994·Advances in Space Research : the Official Journal of the Committee on Space Research (COSPAR)·J M JessupG Spaulding
Mar 1, 1995·Hearing Research·T S NairA L Nuttall
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·I VirtanenJ Ylänne
Jul 1, 1991·Trends in Cell Biology·V Quaranta, J C Jones
May 11, 1992·The Journal of Cell Biology·E C LeeA M Mercurio
Aug 1, 1992·The Journal of Cell Biology·F G GiancottiE Ruoslahti
Sep 26, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Aliya FatehullahInke S Näthke
Oct 15, 1992·European Journal of Biochemistry·T PikkarainenK Tryggvason
Dec 31, 1997·Molecular Biology of the Cell·W C HoB M Chan
Oct 1, 1990·Current Opinion in Cell Biology·A M Mercurio
Jul 27, 2001·Matrix Biology : Journal of the International Society for Matrix Biology·M SundT Pihlajaniemi
Nov 30, 2010·Expert Opinion on Therapeutic Patents·Aadilah OmarStefan F T Weiss
Apr 1, 1994·Cell Adhesion and Communication·A S ClarkeA M Mercurio
Aug 1, 1992·The British Journal of Dermatology·V A VenningF Wojnarowska
May 1, 1997·Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics·A FranchiR Bondi
Jul 10, 2009·BMC Cancer·Anders Bondo DydensborgJean-François Beaulieu

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