Human colon cell culture models of different transformation stages to assess conjugated linoleic acid and conjugated linolenic acid metabolism: Challenges and chances

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Christian DegenGerhard Jahreis

Abstract

Both cellular transformation status and cell culture conditions affect fatty acid metabolism. Hence, the incorporation and metabolism of c9,t11-CLA (conjugated linoleic acid) and other CFAs (conjugated fatty acids) were compared in colon cells (LT-97, adenoma; HT-29, adenocarcinoma). Growth inhibition by CFA in LT-97 cells was assessed via the DAPI (4',6-diamidino-2-phenylindole dihydrochloride) assay. Basal gene expression of desaturases (Δ5, Δ6 and Δ9) and elongases (1, 2, 5 and 6) was determined in LT-97 using PCR. Analysis of cellular fatty acids revealed a 2-fold higher incorporation of c9,t11-CLA (40 and 80μM) in HT-29 cells compared to LT-97 cells. The β-oxidized and elongated conjugated dienoic (CD) fatty acids differed by 8-fold (CD-C16:2/CD-C20:2; HT-29: 8:1; LT-97: 1:1). Notably, LT-97 cells were shown to convert conjugated linolenic acid (CLnA) to CLA. Moreover, LT-97 cells revealed no basal expression of elongase 2. CLnA caused stronger growth inhibition (≤80μM) compared to CLA (200μM). The results indicate that LT-97 cells represent a superior model to carry out elongation and desaturation studies of unsaturated and conjugated fatty acids compared to HT-29 cells. Nevertheless, further in-depth metabolic and transc...Continue Reading

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Citations

Jul 31, 2013·Acta Cirúrgica Brasileira·Antônio Frederico de Freitas GomidesMaria do Carmo Gouveia Peluzio
Jan 11, 2017·Scientific Reports·Stefan SchusterSeverin Sasso
Jan 30, 2019·Journal of the Science of Food and Agriculture·Jesus Rodríguez-MirandaCecilia E Martínez-Sánchez

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