Human colon tissue in organ culture: preservation of normal and neoplastic characteristics.

In Vitro Cellular & Developmental Biology. Animal
Michael K DameJames Varani

Abstract

Normal and neoplastic human colon tissue obtained at surgery was used to establish conditions for organ culture. Optimal conditions included an atmosphere of 5% CO2 and 95% O2; tissue partially submerged with mucosa at the gas interface; and serum-free medium with 1.5 mM Ca2+ and a number of growth supplements. Histological, histochemical, and immunohistochemical features that distinguish normal and neoplastic tissue were preserved over a 2-d period. With normal tissue, this included the presence of elongated crypts with small, densely packed cells at the crypt base and mucin-containing goblet cells in the upper portion. Ki67 staining, for proliferating cells, was confined to the lower third of the crypt, while expression of extracellular calcium-sensing receptor was seen in the upper third and surface epithelium. E-cadherin and β-catenin were expressed throughout the epithelium and confined to the cell surface. In tumor tissue, the same disorganized, abnormal glandular structures seen at time zero were present after 2 d. The majority of cells in these structures were mucin-poor, but occasional goblet cells were seen and mucin staining was present. Ki67 staining was seen throughout the abnormal epithelium and calcium-sensing re...Continue Reading

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Citations

Nov 26, 2010·In Vitro Cellular & Developmental Biology. Animal·Michael K DameJames Varani
Dec 25, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Michael K DameD Kim Turgeon
Jun 21, 2012·Gut Microbes·Katerina Tsilingiri, Maria Rescigno
Aug 1, 2016·Journal of Immunological Methods·Ilaria RussoCarolina Ciacci
Jun 4, 2020·Cancers·George M RamzyPatrycja Nowak-Sliwinska
Jul 29, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elizabeth H FernandoWallace K MacNaughton

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