Abstract
Normal and neoplastic human colon tissue obtained at surgery was used to establish conditions for organ culture. Optimal conditions included an atmosphere of 5% CO2 and 95% O2; tissue partially submerged with mucosa at the gas interface; and serum-free medium with 1.5 mM Ca2+ and a number of growth supplements. Histological, histochemical, and immunohistochemical features that distinguish normal and neoplastic tissue were preserved over a 2-d period. With normal tissue, this included the presence of elongated crypts with small, densely packed cells at the crypt base and mucin-containing goblet cells in the upper portion. Ki67 staining, for proliferating cells, was confined to the lower third of the crypt, while expression of extracellular calcium-sensing receptor was seen in the upper third and surface epithelium. E-cadherin and β-catenin were expressed throughout the epithelium and confined to the cell surface. In tumor tissue, the same disorganized, abnormal glandular structures seen at time zero were present after 2 d. The majority of cells in these structures were mucin-poor, but occasional goblet cells were seen and mucin staining was present. Ki67 staining was seen throughout the abnormal epithelium and calcium-sensing re...Continue Reading
References
Oct 1, 1978·In Vitro·H AutrupB F Trump
Nov 1, 1991·Cytotechnology·J H ResauS J Meltzer
Jan 1, 1985·Annual Review of Physiology·M Lipkin
Oct 1, 1972·Cancer·M Kalus
Aug 1, 1969·The Journal of Clinical Investigation·T H Browning, J S Trier
Jan 1, 1980·Methods in Cell Biology·H Autrup
Nov 1, 1994·The Journal of Clinical Investigation·J VaraniJ J Voorhees
Sep 1, 1993·Biochemical Pharmacology·P Schmiedlin-RenP B Watkins
Sep 1, 1996·The Journal of Pathology·M MoorghenD R Appleton
Feb 11, 2000·Annals of the New York Academy of Sciences·J A BaronE R Greenberg
Dec 20, 2002·Gastroenterology Clinics of North America·Lawrence J Burgart
Nov 10, 2005·British Journal of Cancer·N BhagavathulaJ Varani
Sep 12, 2006·Virology·D Kolodkin-GalA Panet
Nov 30, 2006·The Journal of Gene Medicine·Filippos KesisoglouEllen M Zimmermann
Jun 9, 2007·International Journal of Cancer. Journal International Du Cancer·Narasimharao BhagavathulaJames Varani
Sep 1, 2007·Toxicologic Pathology·James VaraniRobert Dunstan
Mar 5, 2008·Oncogene·V GouyerG Huet
Jun 24, 2008·In Vitro Cellular & Developmental Biology. Animal·Michael K DameJames Varani
Aug 30, 2008·Cancer Letters·James F Whitfield
Feb 14, 2009·Journal of Gastroenterology·Makoto Mark Taketo
Mar 6, 2009·Gastroenterology·Makoto Mark Taketo, Winfried Edelmann
Apr 28, 2009·Cancer Letters·Muhammad Nadeem AslamJames Varani
Jun 19, 2009·In Vitro Cellular & Developmental Biology. Animal·Michael K DameJames Varani
Citations
Nov 26, 2010·In Vitro Cellular & Developmental Biology. Animal·Michael K DameJames Varani
Jun 7, 2012·Genes & Nutrition·Christine BäuerlGaspar Pérez Martínez
Mar 4, 2014·PloS One·Saravanan AyyaduraiDidier Merlin
Dec 25, 2013·Laboratory Investigation; a Journal of Technical Methods and Pathology·Michael K DameD Kim Turgeon
Mar 15, 2015·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Bapi PaharArpita Das
Oct 8, 2015·Science Signaling·Huabing ZhangYatrik M Shah
Jul 23, 2013·Cytokine·Diganta PanBapi Pahar
Jun 21, 2012·Gut Microbes·Katerina Tsilingiri, Maria Rescigno
Aug 1, 2016·Journal of Immunological Methods·Ilaria RussoCarolina Ciacci
Jun 4, 2016·BioFactors·Vasudevan SekarSudhandiran Ganapasam
Jan 10, 2012·The Journal of Reproduction and Development·Yuji HiraoNaoki Takenouchi
Jun 4, 2020·Cancers·George M RamzyPatrycja Nowak-Sliwinska
Feb 20, 2014·Physiological Genomics·Brandon MapesSonia S Kupfer
Sep 29, 2017·Frontiers in Medicine·Rafia S Al-LamkiJordan S Pober
Jul 29, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elizabeth H FernandoWallace K MacNaughton
Oct 18, 2020·International Journal of Molecular Sciences·David Sánchez PeñarandaSilvia Martínez Llorens
Oct 20, 2020·Molecular Therapy Oncolytics·Jérémy BéguinPhilippe Erbs