Human colorectal cancer-derived carcinoma associated fibroblasts promote CD44-mediated adhesion of colorectal cancer cells to endothelial cells by secretion of HGF

Cancer Cell International
Rongsheng ZhangYongdong Feng

Abstract

Carcinoma-associated fibroblasts (CAFs) are dominant components of tumor microenvironment, which has been reported to promote development, progression, and metastasis of cancer. However, the role of CAFs during adhesion process remains unknown. It has been hypothesized that CAFs contribute to adhesion to endothelial cells of colorectal cancer (CRC) via HGF/c-Met pathway. Clinical specimen and orthotopic liver metastasis model was used to investigate association between CD44 expression and propensity of metastasis in CRC. Human CRC derived cancer associated fibroblasts was isolated and its effect on migration and adhesion of CRC cells was investigated. We also confirm the conclusion on animal metastasis model. In this study, clinical specimen and orthotopic liver metastatic model indicated that overexpression of CD44 is associated with CRC metastasis, and we found that colorectal cancer-derived CAFs (CC-CAFs) increased the adhesion and migration of CRC cells in vitro through up-regulation of CD44, we also found that CC-CAFs promoted adhesion and liver or lung metastasis in vivo. Mechanistically, we found that the expression of HGF increased tenfolds compared CC-CAFs with adjacent normal fibroblasts, and HGF promoted adhesion thr...Continue Reading

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Citations

Oct 1, 2019·Liver International : Official Journal of the International Association for the Study of the Liver·Jannis KountourasStergios A Polyzos
Sep 26, 2020·Frontiers in Oncology·Carlos A Gonzalez-VillarrealElsa N Garza-Treviño
Sep 29, 2020·Frontiers in Oncology·Margaux SalaFrédéric Saltel
Jun 11, 2021·Signal Transduction and Targeted Therapy·Fanglong WuHongmei Zhou
Jul 23, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·Arian Ansardamavandi, Mohammad Tafazzoli-Shadpour
Nov 23, 2021·Pharmaceutical Development and Technology·Adel F AlghaithAwwad A Radwan

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Methods Mentioned

BETA
transfection
fluorescence-activated cell sorting
fluorescence microscopy
flow cytometry
enzyme-linked immunosorbent assay
ELISA
PCR
fluorescence

Software Mentioned

SPSS
FlowJo
Treestar

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