Human connexin43 gap junction channels. Regulation of unitary conductances by phosphorylation

Circulation Research
A P MorenoD C Spray

Abstract

Connexin43 is the major gap protein in the heart and cardiovascular system. Single channel recordings of human connexin43 gap junction channels exogenously expressed in transfected SKHep1 cells demonstrate two discrete classes of channel events, with unitary conductances of predominantly 60 to 70 and 90 to 100 pS when recorded with an internal solution containing CsCl as the major current-carrying ionic species and at moderate transjunctional voltages (< 60 mV). Human connexin43 expressed in SKHep1 cells displays multiple electrophoretic mobilities (apparent M(r), approximately 41 to 45 kD) when resolved in Western blots. Treatment of connexin43 from these cells with alkaline phosphatase collapses the bands into a single 41-kD species; application of alkaline phosphatase to the cell interior through patch pipettes yields channels that are predominantly of the larger unitary conductance. The smaller 60- to 70-pS unitary conductance values correspond to the most common channel size seen in cultured rat cardiac myocytes; these channels were more frequently observed after treatment with the phosphatase inhibitor okadaic acid, which was shown to increase phosphorylation of human connexin43 in these cells under similar conditions. Ex...Continue Reading

References

Jun 1, 1992·The American Journal of Physiology·B R Takens-KwakA C Van Ginneken
Sep 3, 1991·Biochimica Et Biophysica Acta·S Y OhA W Murray
Aug 1, 1991·Biophysical Journal·I R Josephson, N Sperelakis
Dec 24, 1991·Brain Research·D C SprayR Dermietzel
Aug 1, 1990·The Journal of Cell Biology·G I FishmanL A Leinwand
Jan 1, 1991·Molecular and Cellular Biology·R KadleB J Nicholson
Mar 1, 1991·Neuron·M V BennettJ C Sáez
Jan 1, 1990·Anatomy and Embryology·R DermietzelD S Spray
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B EghbaliD C Spray
Sep 2, 1985·Nature·J Neyton, A Trautmann
Jan 1, 1985·The Journal of Membrane Biology·R WernerG Dahl
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J M Burt, D C Spray
Oct 1, 1989·Pflügers Archiv : European journal of physiology·A Rüdisüli, R Weingart
Mar 31, 1989·Biochemical and Biophysical Research Communications·H IshiharaD Uemura
Dec 1, 1987·The Journal of Cell Biology·E C BeyerD A Goodenough
Jul 1, 1986·The Journal of Cell Biology·D L Paul
Jan 30, 1987·Biochemical and Biophysical Research Communications·H KaseM Kaneko
Nov 1, 1985·The American Journal of Physiology·R L WhiteM V Bennett
Sep 1, 1984·Cellular and Molecular Neurobiology·M R Costa, W A Catterall

❮ Previous
Next ❯

Citations

Sep 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R BruzzoneD A Goodenough
Sep 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D B Gros, H J Jongsma
Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R J StockertE L Hertzberg
Sep 1, 1995·Pflügers Archiv : European journal of physiology·B R KwakH J Jongsma
Aug 1, 1996·Journal of Bioenergetics and Biomembranes·T W White, R Bruzzone
Jun 15, 2007·The Journal of Membrane Biology·Lene Nygaard AxelsenMorten Schak Nielsen
Sep 12, 2012·The Journal of Membrane Biology·Siddhartha S MitraBruce J Nicholson
Apr 29, 2005·Brain Research. Molecular Brain Research·Xiaosen OuyangJohn O'Brien
Feb 5, 2003·Biology of the Cell·Véronique Cruciani, Svein Ole Mikalsen
Apr 1, 1996·Current Opinion in Structural Biology·M Yeager, B J Nicholson
May 1, 1998·Trends in Cardiovascular Medicine·E Kardami, B W Doble
Oct 16, 2001·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·S Kanno, J E Saffitz
Jul 21, 2007·Visual Neuroscience·W Wade KothmannJohn O'Brien
Aug 23, 2007·Visual Neuroscience·Stephen L MillsDavid W Marshak
Jan 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Dennis RottlaenderUta C Hoppe
Apr 1, 1997·The Journal of General Physiology·H Z Wang, R D Veenstra
Apr 1, 1995·Molecular Biology of the Cell·T W WhiteR Bruzzone
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Daniel A Goodenough, David L Paul
May 15, 1996·European Journal of Biochemistry·R BruzzoneD L Paul
Jun 1, 1995·Journal of Cardiovascular Electrophysiology·J E SaffitzE C Beyer
Feb 1, 1995·Journal of Cardiovascular Electrophysiology·L M DavisE C Beyer
Aug 1, 1997·Journal of Cardiovascular Electrophysiology·G E MorleyM Delmar
Oct 9, 1999·Journal of Cardiovascular Electrophysiology·W C De Mello
Jan 15, 2000·Journal of Cardiovascular Electrophysiology·J JalifeD Vaidya
Oct 22, 2002·American Journal of Physiology. Cell Physiology·G Trevor CottrellJanis M Burt
May 12, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Rebecca L McKinnonKarel Tyml
May 4, 2005·Circulation·Atsushi SanbeJeffrey Robbins
Apr 30, 2011·Circulation Research·Benjamin F RemoGlenn I Fishman
Jan 19, 2013·Cardiovascular Diabetology·Kristine Boisen OlsenMorten Schak Nielsen
Sep 8, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R Tonon, P D'Andrea
Aug 14, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M Romanello, P D'Andrea
Jun 3, 2006·Biological Chemistry·Xianrong ZhangYipeng Qi
Mar 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicolás Palacios-PradoFeliksas F Bukauskas
May 8, 2003·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·James L BorkeCarlos M Isales
Dec 29, 2011·Frontiers in Bioscience (Scholar Edition)·Julio Cesar Batista FerreiraMohamed Boutjdir

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.