Human cyclin B3. mRNA expression during the cell cycle and identification of three novel nonclassical nuclear localization signals

The FEBS Journal
Katrin TschöpKurt Engeland

Abstract

Cyclins form complexes with cyclin-dependent kinases. By controlling activity of the enzymes, cyclins regulate progression through the cell cycle. A- and B-type cyclins were discovered due to their distinct appearance in S and G(2) phases and their rapid proteolytic destruction during mitosis. Transition from G(2) to mitosis is basically controlled by B-type cyclins. In mammals, two cyclin B proteins are well characterized, cyclin B1 and cyclin B2. Recently, a human cyclin B3 gene was described. In contrast to the expression pattern of other B-type cyclins, we find cyclin B3 mRNA expressed not only in S and G(2)/M cells but also in G(0) and G(1). Human cyclin B3 is expressed in different variants. We show that one isoform remains in the cytoplasm, whereas the other variant is translocated to the nucleus. Transport to the nucleus is dependent on three autonomous nonclassical nuclear localization signals that where previously not implicated in nuclear translocation. It had been shown that cyclin B3 coimmunoprecipitates with cdk2; but this complex does not exhibit any kinase activity. Furthermore, a degradation-resistant version of cyclin B3 can arrest cells in G(1) and G(2). Taken together with the finding that cyclin B3 mRNA is ...Continue Reading

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Citations

Mar 17, 2009·Journal of Assisted Reproduction and Genetics·Ann A KiesslingDimitris Loutradis
Nov 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Daniel E ConwayLarry V McIntire
Jan 21, 2015·Biochemical and Biophysical Research Communications·Hirokazu Shiheido, Jun Shimizu
Jan 15, 2009·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Virak VisudtipholeKanyawim Kirtikara
Mar 4, 2010·Molecular Reproduction and Development·Jean-Claude LozanoGérard Peaucellier
Jan 18, 2007·The Plant Journal : for Cell and Molecular Biology·Kevin M CulliganAnne B Britt
Mar 2, 2019·Science China. Life Sciences·Jing XuShan Gao

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