PMID: 8985337Jan 1, 1997Paper

Human cytomegalovirus capsid assembly protein precursor (pUL80.5) interacts with itself and with the major capsid protein (pUL86) through two different domains

Journal of Virology
L J WoodW Gibson

Abstract

We have used the yeast GAL4 two-hybrid system to examine interactions between the human cytomegalovirus (HCMV) major capsid protein (MCP, encoded by UL86) and the precursor assembly protein (pAP, encoded by UL80.5 and cleaved at its carboxyl end to yield AP) and found that (i) the pAP interacts with the MCP through residues located within the carboxy-terminal 21 amino acids of the pAP, called the carboxyl conserved domain (CCD); (ii) the pAP interacts with itself through a separate region, called the amino conserved domain (ACD), located between amino acids His34 and Arg52 near the amino end of the molecule; (iii) the simian CMV (SCMV) pAP and AP can interact with or replace their HCMV counterparts in these interactions, whereas the herpes simplex virus pAP and AP homologs cannot; and (iv) the HCMV and SCMV maturational proteinase precursors (ACpra, encoded by UL80a and APNG1, respectively) can interact with the pAP and MCP. The ACD and CCD amino acid sequences are highly conserved among members of the betaherpesvirus group and appear to have counterparts in the alpha- and gammaherpesvirus pAP homologs. Deleting the ACD from the HCMV pAP, or substituting Ala for a conserved Leu in the ACD, eliminated detectable pAP self-interac...Continue Reading

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Citations

Jul 5, 2002·Journal of Virology·David A McClellandFrazer J Rixon
Jan 29, 2011·The Journal of General Virology·Kyosuke KanaiNaoki Inoue
Nov 2, 2014·The Journal of General Virology·Blair L Strang
May 29, 1999·The Journal of Biological Chemistry·B Greene, J King
Nov 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Eain MurphyThomas E Shenk
Oct 22, 2010·Journal of Virology·Stacia L Phillips, Wade A Bresnahan
Jul 29, 1998·Journal of Molecular Biology·M H ParkerP E Prevelige
Aug 1, 1997·Current Opinion in Chemical Biology·D L FlynnB C Holwerda
Jun 1, 1997·Antiviral Research·B C Holwerda
Dec 28, 2005·Journal of Virology·Mercy E OkoyePrashant Desai
Nov 5, 2002·Journal of Virology·Catherine A Blankenship, Thomas Shenk

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