Human cytomegalovirus carries a cell-derived phospholipase A2 required for infectivity

Journal of Virology
Cuider AllalJean-Luc Davignon

Abstract

Human cytomegalovirus (HCMV) is known to carry host cell-derived proteins and mRNAs whose role in cell infection is not understood. We have identified a phospholipase A2 (PLA2) activity borne by HCMV by using an assay based on the hydrolysis of fluorescent phosphatidylcholine. This activity was found in all virus strains analyzed and in purified strains. It was calcium dependent and was sensitive to inhibitors of cytosolic PLA2 (cPLA2) but not to inhibitors of soluble PLA2 or calcium-independent PLA2. No other phospholipase activity was detected in the virus. Purified virus was found to contain human cellular cPLA2alpha, as detected by monoclonal antibody. No homology with PLA2 was found in the genome of HCMV, indicating that HCMV does not code for a PLA2. Decreased de novo expression of immediate-early proteins 1 and 2 (IE1 and IE2), tegument phosphoprotein pp65, and virus production was observed when HCMV was treated with inhibitors of cPLA2. Cell entry of HCMV was not altered by those inhibitors, suggesting the action of cPLA2 was postentry. Together, our results indicate a selective sorting of a cell-derived cPLA2 during HCMV maturation, which is further required for infectivity.

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Citations

May 14, 2005·Journal of Virology·Réjean CantinMichel J Tremblay
May 14, 2005·Journal of Virology·Jeremy P KamilHsing-Jien Kung
Mar 30, 2012·PLoS Pathogens·Rushika PereraRichard J Kuhn
Jul 21, 2010·Cellular Microbiology·Amanda Linkous, Eugenia Yazlovitskaya
Jan 13, 2006·The Journal of Biological Chemistry·Javier CasasMaría A Balboa
Jan 9, 2021·Arthritis Research & Therapy·Jean-Luc DavignonAlain Cantagrel
Jul 23, 2020·International Journal of Biological Macromolecules·S C TeixeiraD S Lopes
Jul 2, 2021·Antiviral Research·Andrea J ParsonsDomenico Tortorella
May 13, 2010·Molecular Pharmaceutics·Huong T LeJeffrey A Hughes
Aug 28, 2021·International Journal of Molecular Sciences·Pierre LayrolleStéphane Chavanas

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