Human cytomegalovirus early infection, acute rejection, and major histocompatibility class II expression in transplanted lung. Molecular, immunocytochemical, and histopathologic investigations

Transplantation
E ArbustiniE Solcia

Abstract

The present study aimed to investigate the relationship between acute rejection and human cytomegalovirus (HCMV) infection, as well as the coexpression of HLA-DR and immediate-early (IE) viral antigens, in 143 transbronchial biopsies and bronchoalveolar lavage fluids of 32 lung transplant recipients. We investigated the occurrence of morphologically overt viral infection with conventional histopathology, the expression of IE antigens with single labeling immunohistochemistry, the coexpression of IE antigens and HLA-DR molecules with double labeling techniques, and the presence of viral IE genes with polymerase chain reaction. Histopathologic study showed overt viral infections (12.6%) in 18 of the 143 biopsies; 8 were in a context of pneumonia and 10 were localizations without surrounding inflammatory cells; immunohistochemistry showed IE viral antigen expression in 31 (21.67%); PCR detected viral IE genes in 73/143 lavage fluids and biopsies (51%). The double labeling immunohistochemical technique showed that most IE antigen-expressing, noncytopathic cells were either HLA-DR negative in areas without infiltrates, or HLA-DR positive in those areas where inflammatory infiltrates were consistent, in the absence of viral cytopathy...Continue Reading

References

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Citations

Mar 21, 2008·Acta Neuropathologica·Michael E ScheurerRanda El-Zein
Sep 10, 2002·Transplant Immunology·Benjamin A Bradley
Nov 2, 1999·Transplant Immunology·A T BorchersM E Gershwin
Mar 14, 2003·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Heyman LuckrazJohn Wallwork
Sep 6, 2003·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Heyman LuckrazStephen R Large
May 2, 2001·Transplantation·J Kurtz
Sep 11, 2002·The Lancet Infectious Diseases·Francesca Cainelli, Sandro Vento
Jun 30, 2009·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Peter JakschWalter Klepetko
Jun 1, 1997·Clinics in Chest Medicine·K Kelly, M I Hertz
Nov 27, 2002·Transplantation·Eliane R PopaMartin C Harmsen

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