Human cytomegalovirus major immediate-early proteins and simian virus 40 large T antigen can inhibit apoptosis through activation of the phosphatidylinositide 3'-OH kinase pathway and the cellular kinase Akt

Journal of Virology
Yongjun Yu, James C Alwine

Abstract

The temperature-sensitive cell line ts13 is mutated in CCG1, the gene encoding TAF(II)250, the largest of the TATA-binding protein-associated factors (TAFs) in TFIID. At the nonpermissive temperature, the temperature-sensitive phenotypes are (i) transcription defects, (ii) cell cycle arrest in G(1), and (iii) apoptosis. We previously demonstrated that the human cytomegalovirus (HCMV) major immediate-early proteins (MIEPs) can rescue the transcription defects and inhibit apoptosis at the nonpermissive temperature. In the work presented, we show that activation of the cellular kinase Akt alone can inhibit apoptosis in ts13 cells grown at the nonpermissive temperature. More significantly, we show that the HCMV MIEPs can activate Akt, resulting in the inhibition of apoptosis. In parallel experiments, we found that simian virus 40 (SV40) large T antigen can mediate the same function. These experiments were done by transfecting the HCMV major immediate-early gene or a cDNA encoding T antigen into ts13 cells, and thus neither viral attachment to receptors, viral tegument proteins, nor any other viral protein is required for Akt activation. Akt is activated by the phosphatidylinositide 3'-OH (PI3) kinase pathway. Using a specific inhib...Continue Reading

References

Sep 1, 1977·Journal of Cellular Physiology·A Talavera, C Basilico
Jul 1, 1984·The Journal of General Virology·M P Landini
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·T F KowalikE S Huang
Jun 1, 1996·Genes & Development·B Damania, J C Alwine
Oct 6, 1997·Journal of Virology·D M LukacJ C Alwine
Aug 1, 1998·The Journal of Biological Chemistry·M Pap, G M Cooper
Feb 13, 1999·Cell·I Conlon, M Raff
May 18, 1999·Molecular and Cellular Biology·Q WangA Klip
Jun 9, 1999·Current Biology : CB·L P KaneA Weiss
Oct 19, 1999·Molecular and Cellular Biology·M M HillS L Macaulay
Dec 2, 1999·Genes & Development·S R DattaM E Greenberg
Aug 10, 2000·Journal of Virology·J P CastilloT F Kowalik
Aug 4, 2001·Molecular and Cellular Biology·M G Vander HeidenC B Thompson

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Citations

Apr 9, 2010·Medical Microbiology and Immunology·Elita AvotaSibylle Schneider-Schaulies
Oct 4, 2007·Transgenic Research·Racheli SteinbergShira Neuman-Silberberg
Apr 3, 2007·Journal of Biomedical Science·Hsueh-Hsuan LinNing-Hsing Yeh
Apr 14, 2010·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Robert Saddawi-Konefka, John R Crawford
Jan 15, 2004·Trends in Molecular Medicine·Jindrich CinatlHans Wilhelm Doerr
Jul 12, 2002·Trends in Microbiology·Edward S Mocarski
Apr 23, 2003·Trends in Microbiology·Martin ScholzJindrich Cinatl
Mar 4, 2008·Nature Reviews. Microbiology·Nicholas J BuchkovichJames C Alwine
Nov 22, 2005·Oncogene·Clodagh C O'Shea
Mar 21, 2012·Proceedings of the National Academy of Sciences of the United States of America·Caleb McKinneyIan Mohr
Mar 4, 2011·The Journal of Biological Chemistry·Daisheng Song, Frank S Lee
Oct 26, 2012·The Journal of Biological Chemistry·Zhe LiuJing-hsiung James Ou
Dec 30, 2008·The Journal of Biological Chemistry·Karen A UrtishakEric J Brown
Feb 10, 2012·Neuro-oncology·Kristine DziurzynskiUNKNOWN HCMV and Gliomas Symposium
Jan 5, 2007·Journal of Virology·Scott TerhuneDong Yu
Sep 23, 2011·Journal of Virology·Carisa TiltonJames C Alwine
Jul 16, 2004·Journal of Virology·Sita AwasthiJames C Alwine
Jan 30, 2004·Journal of Virology·Christine C Tomlinson, Blossom Damania
Apr 28, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jun CaoLeda Raptis
Sep 29, 2011·The Journal of Clinical Investigation·Ninib BaryawnoCecilia Söderberg-Nauclér
Dec 11, 2013·Infectious Agents and Cancer·Kenneth AlibekSamal Zhussupbekova
Apr 7, 2011·Experimental & Molecular Medicine·Chang Hoon Cho
Sep 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sagar B KudchodkarJames C Alwine
Jun 9, 2009·Bioscience, Biotechnology, and Biochemistry·Zhiqiang BaiXuxia Song
Dec 20, 2013·Viruses·Nora Diehl, Heiner Schaal
Jan 31, 2004·Seminars in Immunology·Fumihiko TakeshitaDennis M Klinman
Oct 26, 2010·Virus Research·Kavitha DhuruvasanPhilip E Pellett
Oct 26, 2010·Virus Research·Wolfram Brune
Apr 11, 2009·Transplantation Proceedings·L A Tibbles
Jun 30, 2011·Reviews in Medical Virology·Robert van Domselaar, Niels Bovenschen

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