Human cytomegalovirus UL97 kinase alters the accumulation of CDK1.

The Journal of General Virology
Rachel B GillM N Prichard

Abstract

The UL97 protein kinase is a serine/threonine kinase expressed by human cytomegalovirus (CMV) that phosphorylates ganciclovir. An investigation of the subcellular localization of pUL97 in infected cells indicated that, early in infection, pUL97 localized to focal sites in the nucleus that transitioned to subnuclear compartments and eventually throughout the entire nucleus. When UL97 kinase activity was eliminated with a K355M mutation or pharmacologically inhibited with maribavir, the expansion and redistribution of pUL97 foci within the nucleus was delayed, nuclear reorganization did not occur and assembly complexes in the cytoplasm failed to form normally. As UL97 kinase and its homologues appear to be functionally related to CDK1, a known regulator of nuclear structural organization, the effects of the UL97 kinase on CDK1 were investigated. Expression of CDK1 in infected cells appeared to be induced by UL97 kinase activity at the level of transcription and was not tied to other virus life-cycle events, such as viral DNA replication or virion assembly. These results suggest that, in addition to phosphorylating CDK1 targets, the UL97 kinase modifies G₂/M cell-cycle checkpoint regulators, specifically CDK1, to promote virus rep...Continue Reading

References

Feb 1, 1997·Journal of Virology·D Dittmer, E S Mocarski
Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T OnishiK Hruska
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·D G WolfE S Mocarski
Oct 16, 2002·The Journal of Cell Biology·Stephen C Ogg, Angus I Lamond
Apr 1, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sunil J AdvaniBernard Roizman
May 29, 2003·The Journal of Biological Chemistry·Giuseppina CarettiRoberto Mantovani
Jun 25, 2003·Antimicrobial Agents and Chemotherapy·Stephanie L WilliamsEarl R Kern
Jun 28, 2003·Journal of Virology·Paula M KroskyPhiroze B Sethna
Sep 25, 2004·Medicinal Research Reviews·Jindrich CinatlHans Wilhelm Doerr
Sep 29, 2004·Journal of Virology·Susan M VarnumJay A Nelson
Feb 1, 2005·Journal of Virology·Afsar Rahbar, Cecilia Söderberg-Nauclér
Jun 25, 2005·The Journal of Biological Chemistry·Manfred MarschallThomas Stamminger
Aug 30, 2005·Antimicrobial Agents and Chemotherapy·Stephanie L Williams-AzizEarl R Kern
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Mark N PrichardEarl R Kern
Jun 13, 2006·Antiviral Research·Karen K Biron

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Citations

Jun 28, 2014·Molecular & Cellular Proteomics : MCP·Jens MilbradtManfred Marschall

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