PMID: 8964983Oct 1, 1996Paper

Human delayed-type hypersensitivity reaction in a SCID mouse engrafted with human T cells and autologous skin

The Journal of Investigative Dermatology
P PetzelbauerK Wolff

Abstract

We have developed and animal model to study human delayed-type hypersensitivity reactions occurring in a human environment within a mouse host. Human skin was grafted onto the backs and autologous human immune cells were injected into the peritoneal cavity of mice with severe combined immunodeficiency. Seven and 14 d after grafting, 2-50% of total white blood and spleen cells were of human origin. Mouse spleen-derived human T cells from tetanus toxoid-sensitized donors proliferated in response to tetanus toxoid as measured by [3H]thymidine uptake, and the strength of this proliferative response equaled that with pre-graft T cells from the same donor. Proliferation was blocked with monoclonal antibodies to human but not to mouse major histocompatibility complex antigens and with anti-human CD4 monoclonal antibodies. In vivo vaccination of mice with tetanus toxoid did not enhance proliferation of mouse spleen-derived human T cells in response to antigen. Injection of tetanus toxoid into the human skin graft caused a perivascular human CD4+/CD25+ T-cell infiltrate, which was not present when tetanus toxoid was injected into adjacent mouse skin. We conclude that human T cells grafted into mice with severe combined immunodeficiency ...Continue Reading

Citations

Jan 9, 2013·Expert Opinion on Drug Discovery·Pinar AvciMichael R Hamblin
Aug 16, 2005·Experimental Dermatology·Heinfried H RadekeWolf-Henning Boehncke
Apr 19, 2008·Journal of Cellular and Molecular Medicine·Baojun ZhangYong Zhao
May 23, 2015·Scandinavian Journal of Immunology·B C MartelL K Poulsen
Oct 12, 2001·Annals of the New York Academy of Sciences·J S PoberJ S Schechner
Jun 1, 2006·Expert Opinion on Drug Discovery·Frederik H IgneyThomas M Zollner
Jul 16, 2002·Laboratory Investigation; a Journal of Technical Methods and Pathology·Stéphanie SénéchalAnne Tsicopoulos

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.