Abstract
Many studies on tissue stem cells have been conducted in the field of regenerative medicine, and some studies have indicated that cultured dental pulp mesenchymal cells secrete dentin matrix. In the present study we used alginate as a scaffold to transplant subcultured human dental pulp cells subcutaneously into the backs of nude mice. We found that when beta-glycerophosphate was added to the culture medium, dentin sialophosphoprotein mRNA coding dentin sialoprotein (DSP) was expressed. An increase in alkaline phosphatase, which is an early marker for odontoblast differentiation, was also demonstrated. At 6 weeks after implantation the subcutaneous formation of radio-opaque calcified bodies was observed in situ. Immunohistochemical and fine structure studies identified expression of type I collagen, type III collagen, and DSP in the mineralizing transplants. Isolated odontoblast-like cells initiated dentin-like hard tissue formation and scattered autolyzing apoptotic cells were also observed in the transplants. The study showed that subcultured dental pulp cells actively differentiate into odontoblast-like cells and induce calcification in an alginate scaffold.
References
Dec 1, 1992·Archives of Oral Biology·Y TsukamotoM Mori
Jan 1, 1991·Archives of Oral Biology·M Nakashima
Jan 1, 1991·Archives of Oral Biology·D SeuxH Magloire
Dec 1, 1995·Muscle & Nerve·S WakitaniA I Caplan
Jan 1, 1994·Archives of Oral Biology·A J SmithH Lesot
Sep 1, 1993·Archives of Oral Biology·S KasugaiH Ogura
Jul 1, 1996·American Journal of Ophthalmology·H R DesatnikC Y Lowder
Jan 10, 1997·The Journal of Biological Chemistry·M MacDougallT T Gu
Apr 2, 1999·Science·M F PittengerD R Marshak
Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·F BleicherH Magloire
Feb 1, 2000·Calcified Tissue International·M L CoubleH Magloire
Feb 1, 2000·Calcified Tissue International·S YokoseT Katayama
May 29, 2000·Journal of Biomedical Materials Research·H A AwadG P Boivin
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·S GronthosS Shi
Feb 15, 2001·Nature Genetics·P Patel
Feb 15, 2001·Journal of Hematotherapy & Stem Cell Research·M K MajumdarS L Gerson
Apr 30, 2003·Arthritis Research & Therapy·Rocky S TuanRichard Tuli
Oct 17, 2003·European Cells & Materials·E Sachlos, J T Czernuszka
Dec 24, 2003·Journal of Dental Research·E MatalovaP T Sharpe
Jul 15, 2004·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Nobuyuki KamataShigeru Yasumoto
Sep 8, 2004·Advanced Drug Delivery Reviews·Valerie Liu Tsang, Sangeeta N Bhatia
Sep 15, 2004·Tissue Engineering·Bodo KurzMichael Schünke
Feb 3, 2005·Tissue Engineering·C DommB Kurz
Mar 19, 2005·Cytotherapy·L SongR S Tuan
Citations
Apr 10, 2013·Cellular and Molecular Life Sciences : CMLS·P ChatakunM Atari
Jan 15, 2016·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Arezoo JahanbinMohammad Ali Raisolsadat
May 5, 2018·Tissue Engineering. Part B, Reviews·Valérie OrtiIvan Panayotov
Oct 31, 2018·Journal of Tissue Engineering and Regenerative Medicine·Dina G Moussa, Conrado Aparicio
Feb 6, 2017·Biomedizinische Technik. Biomedical Engineering·Christian ApelSabine Neuss
Jun 1, 2012·Okajimas Folia Anatomica Japonica·Yasutomo IwaiShunji Kumabe
Aug 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nicholas G FischerConrado Aparicio
Dec 4, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Hossein E JazayeriLobat Tayebi