PMID: 16526573Mar 11, 2006Paper

Human dental pulp cell culture and cell transplantation with an alginate scaffold

Okajimas Folia Anatomica Japonica
Shunji KumabeYasutomo Iwai

Abstract

Many studies on tissue stem cells have been conducted in the field of regenerative medicine, and some studies have indicated that cultured dental pulp mesenchymal cells secrete dentin matrix. In the present study we used alginate as a scaffold to transplant subcultured human dental pulp cells subcutaneously into the backs of nude mice. We found that when beta-glycerophosphate was added to the culture medium, dentin sialophosphoprotein mRNA coding dentin sialoprotein (DSP) was expressed. An increase in alkaline phosphatase, which is an early marker for odontoblast differentiation, was also demonstrated. At 6 weeks after implantation the subcutaneous formation of radio-opaque calcified bodies was observed in situ. Immunohistochemical and fine structure studies identified expression of type I collagen, type III collagen, and DSP in the mineralizing transplants. Isolated odontoblast-like cells initiated dentin-like hard tissue formation and scattered autolyzing apoptotic cells were also observed in the transplants. The study showed that subcultured dental pulp cells actively differentiate into odontoblast-like cells and induce calcification in an alginate scaffold.

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Citations

Jan 15, 2016·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Arezoo JahanbinMohammad Ali Raisolsadat
May 5, 2018·Tissue Engineering. Part B, Reviews·Valérie OrtiIvan Panayotov
Oct 31, 2018·Journal of Tissue Engineering and Regenerative Medicine·Dina G Moussa, Conrado Aparicio
Feb 6, 2017·Biomedizinische Technik. Biomedical Engineering·Christian ApelSabine Neuss
Aug 5, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Nicholas G FischerConrado Aparicio
Dec 4, 2019·Dental Materials : Official Publication of the Academy of Dental Materials·Hossein E JazayeriLobat Tayebi

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