PMID: 7528242Jan 1, 1995Paper

Human dermal endothelial cells express membrane-associated mast cell growth factor

The Journal of Investigative Dermatology
R R WeissG F Murphy

Abstract

Mast cell growth factor (MGF), a molecule that serves as a ligand for the receptor tyrosine kinase c-kit, is important in mast cell differentiation, migration, and activation. Previous studies of paraffin-embedded human skin using antibody to murine MGF and reverse transcription-polymerase chain reaction have demonstrated MGF protein and mRNA expression in keratinocytes and isolated dermal cells. We utilized a monoclonal antibody to human MGF to further define patterns of immunoreactivity in frozen specimens of neonatal and adult skin from normal individuals and from patients with urticaria pigmentosa. In addition to keratinocytes and isolated dermal cells in normal and urticaria pigmentosa skin, MGF was detected in cells lining superficial and mid-dermal vessels. Co-expression of MGF and the vascular antigen CD31, and immunoelectron microscopy, identified MGF-positive cells as endothelial cells. Patterns of endothelial MGF expression were not influenced by mast cell degranulation and endothelial E-selectin induction in vitro. By ultrastructure, unfixed specimens demonstrated MGF expression both within the endothelial cytoplasm and in association with lumenal, but not ablumenal, surfaces. Specimens fixed with Nakane's solution ...Continue Reading

Citations

Jan 20, 1999·The British Journal of Dermatology·M C KoeppelJ Sayag
Sep 20, 2000·The Journal of Experimental Medicine·C T MierkeS C Bischoff
Sep 13, 2002·American Journal of Veterinary Research·Maria José RegueraRosa M Rabanal
May 15, 2013·Cellular & Molecular Biology Letters·Kamonnaree ChotinantakulWilairat Leeanansaksiri
Aug 19, 1997·Proceedings of the National Academy of Sciences of the United States of America·B J LongleyN M Schechter
Jun 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·K Norrby
Feb 13, 2001·Blood·M HartmanF Levi-Schaffer
Mar 4, 2000·The American Journal of Dermatopathology·M J RegueraL Ferrer
Jun 12, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Laurence J Walsh
Dec 26, 2015·International Journal of Molecular Sciences·Amber N MullYu-Chieh Wang
Sep 24, 2005·Histopathology·A M Ahmed El-Refaie, A D Burt
Dec 1, 2001·Human Pathology·I EspositoM W Büchler
Apr 28, 2005·The Journal of Investigative Dermatology·Hidehiko BabaShinichi Watanabe

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