Human Dermal Fibroblast Viability and Adhesion on Cellulose Nanomaterial Coatings: Influence of Surface Characteristics.

Biomacromolecules
Ruut KummalaMartti Toivakka

Abstract

Biodegradable and renewable materials, such as cellulose nanomaterials, have been studied as a replacement material for traditional plastics in the biomedical field. Furthermore, in chronic wound care, modern wound dressings, hydrogels, and active synthetic extracellular matrices promoting tissue regeneration are developed to guide cell growth and differentiation. Cells are guided not only by chemical cues but also through their interaction with the surrounding substrate and its physicochemical properties. Hence, the current work investigated plant-based cellulose nanomaterials and their surface characteristic effects on human dermal fibroblast (HDF) behavior. Four thin cellulose nanomaterial-based coatings produced from microfibrillar cellulose (MFC), cellulose nanocrystals (CNC), and two TEMPO-oxidized cellulose nanofibers (CNF) with different total surface charge were characterized, and HDF viability and adhesion were evaluated. The highest viability and most stable adhesion were on the anionic CNF coating with a surface charge of 1.14 mmol/g. On MFC and CNC coated surfaces, HDFs sedimented but were unable to anchor to the substrate, leading to low viability.

References

Jan 1, 1989·The Journal of Investigative Dermatology·B CoulombL Dubertret
May 11, 2005·The Journal of Cell Biology·Hui ChenSusan W Craig
May 11, 2010·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Amnon BuxboimDennis E Discher
Oct 6, 2010·ACS Applied Materials & Interfaces·Khaled A MahmoudJohn H T Luong
May 14, 2011·Chemical Society Reviews·Robert J MoonJeff Youngblood
May 21, 2011·Angewandte Chemie·Dieter KlemmAnnie Dorris
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Sep 12, 2012·Acta Biomaterialia·James M DuganStephen J Eichhorn
Feb 12, 2013·Nanomedicine·James M DuganStephen J Eichhorn
May 14, 2014·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Panagiotis KrontirasDaniel A Hägg
May 27, 2014·Colloids and Surfaces. B, Biointerfaces·Zahid HanifSoong Ho Um
Jul 6, 2014·Journal of Pharmaceutical Sciences·Naeema MayetViness Pillay
Oct 9, 2014·ACS Applied Materials & Interfaces·Nicole E ZanderJohn T Grant
Aug 8, 2015·International Journal of Molecular Sciences·Amelia Ahmad Khalili, Mohd Ridzuan Ahmad
Mar 2, 2016·Current Opinion in Biotechnology·Tiffany AbitbolOded Shoseyov
Apr 29, 2016·Nature Methods·Steven R Caliari, Jason A Burdick
Jun 18, 2016·Carbohydrate Polymers·Henriette Rogstad NordliBrita Pukstad
Aug 6, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·T HakkarainenH Kavola
Apr 13, 2017·Cellular and Molecular Life Sciences : CMLS·Jennifer L Bays, Kris A DeMali
Dec 1, 2017·BMC Bioinformatics·Curtis T RuedenKevin W Eliceiri
Jul 4, 2018·PLoS Biology·Claire McQuinAnne E Carpenter
Oct 3, 2018·ACS Sustainable Chemistry & Engineering·Wenyang XuChunlin Xu
Mar 7, 2019·Microbial Biotechnology·Raquel PortelaRita G Sobral
Jun 6, 2019·Biomaterials Science·Diosangeles Soto VelizMartti Toivakka
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Sara Metwally, Urszula Stachewicz
Oct 1, 2018·Progress in Polymer Science·Shengjie LingDavid L Kaplan

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Methods Mentioned

BETA
transmission electron microscopy
atomic force microscopy
AFM
fluorescence microscopy
chemical treatments
light scattering

Software Mentioned

Image Lab
OneAttension
CellProfiler
JPK Data Processing
sQube
ImageJ
FijiImageJ

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