PMID: 7543547Aug 1, 1995Paper

Human dermal microvascular endothelial cells produce matrix metalloproteinases in response to angiogenic factors and migration

The Journal of Investigative Dermatology
L A CorneliusH G Welgus

Abstract

Matrix metalloproteinases (MMPs) are a family of inducible enzymes that degrade extracellular matrix components, allowing cells to traverse connective tissue structures efficiently. Specific tissue inhibitors (TIMPs) function as physiologic inhibitors of MMP activity. Because neovascularization may require various proteinases, we characterized the profile of metalloenzyme production by microvascular endothelial cells (MEC) and the modulation of expression by phorbol esters (PMA) and by the physiologically relevant cytokines tumor necrosis factor-alpha (TNF-alpha), basic fibroblast growth factor, and interferon-gamma. MMP expression by MEC and large-vessel human umbilical vein endothelial cells (HUVEC) was determined by enzyme-linked immunosorbent assay, immunoprecipitation, Northern hybridization, and transfection assays. Constitutive expression of MMPs by endothelial cells was low. PMA stimulated the production of collagenase, stromelysin, 92-kDa gelatinase, and TIMP-1 in both endothelial cell types. TIMP-2 was constitutively expressed by MEC and HUVEC, but was down-regulated by PMA. TNF-alpha induced an endothelial-cell-specific up-regulation of collagenase with a concomitant inhibition of PMA-induced TIMP-1 up-regulation, a ...Continue Reading

References

Aug 1, 1992·The Journal of Clinical Investigation·S LacrazH G Welgus
Oct 1, 1992·The Journal of Investigative Dermatology·M Ståhle-BäckdahlW C Parks
Nov 1, 1991·Microvascular Research·J A JerdanB M Glaser
Apr 1, 1990·Trends in Genetics : TIG·L M Matrisian
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M Fràter-SchröderP Böhlen
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G I GoldbergC S He
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T CollinsJ S Pober
May 1, 1983·Collagen and Related Research·T W CooperA Z Eisen
Aug 1, 1993·Journal of Cellular Physiology·H W SchnaperD L French
Jan 1, 1993·Kidney International·E DejanaM G Lampugnani

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Citations

Jul 29, 1997·International Journal of Cancer. Journal International Du Cancer·Y NagashimaK Miyazaki
Jul 17, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·D BlankaertJ Werenne
Apr 26, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·W H ZhuR Francesco Nicosia
Apr 18, 2001·Journal of Neuropathology and Experimental Neurology·V HummelP Rieckmann
Mar 7, 2002·American Journal of Physiology. Cell Physiology·Masako YasudaToshinori Yamamoto
Sep 23, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stefania MitolaFederico Bussolino
Sep 24, 2004·Journal of Biomedical Materials Research. Part a·Ping Xu, Michael V Sefton
Aug 11, 2000·In Vitro Cellular & Developmental Biology. Animal·E BonannoR F Nicosia
Jun 16, 1997·FEBS Letters·N KoshikawaK Miyazaki
Nov 19, 2003·Cell Biochemistry and Function·Jacky Arkell, Christopher J Jackson
Sep 11, 2003·Journal of Wound Care·M S Neal
Dec 15, 2006·International Journal of Immunopathology and Pharmacology·O SimonettiA Offidani
Sep 5, 2018·BMC Veterinary Research·Manuela MartanoPaola Maiolino
Nov 9, 2018·Frontiers in Physiology·Qian ZhangXiaojing Wang
Jul 14, 2001·Arteriosclerosis, Thrombosis, and Vascular Biology·M S Pepper
Aug 26, 1998·The British Journal of Surgery·D A McNamaraD J Bouchier-Hayes
Mar 7, 2001·In Vitro Cellular & Developmental Biology. Animal·D K ZablockiB R Alevriadou
Jul 21, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D G StupackD A Cheresh
May 15, 2010·International Journal of Dermatology·Giuseppe MicaliMaria Rita Nasca
Mar 2, 2012·The Journal of Clinical Investigation·Abdessamad ZerrouqiErwin G Van Meir
Jul 10, 2014·International Journal of Immunopathology and Pharmacology·A LeoneA R Jurjus
May 2, 2017·Frontiers in Bioengineering and Biotechnology·Lea KnezevicWolfgang Holnthoner
Jul 6, 2005·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Christian L Baum, Christopher J Arpey
Jan 1, 1996·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·W D ShingletonT E Cawston
Jun 24, 2008·Endothelium : Journal of Endothelial Cell Research·Xi-Qiao WangDa-Zhong Xu
Feb 5, 1999·Equine Veterinary Journal. Supplement·C C PollittS Pollitt
Sep 5, 2002·The British Journal of Dermatology·M BhushanC E M Griffiths
Feb 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·O Behrendtsen, Z Werb
Aug 13, 2002·Plastic and Reconstructive Surgery·Ziv M PeledMichael T Longaker
Feb 7, 2012·The International Journal of Biological Markers·Nataša Todorović-RakovićZaki Abu Rabi

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