Human diploid fibroblast growth on polystyrene microcarriers in aggregates.

Cytotechnology
J VaraniW J Hillegas

Abstract

Polystyrene microcarriers were prepared in four size ranges (53-63 μm, 90-125 μm, 150-180 μm and 300-355 μm) and examined for ability to support attachment and growth of human diploid fibroblasts. Cells attached rapidly to the microcarriers and there was a direct relationship between cell attachment and microcarrier aggregation. Phasecontrast and scanning electron microscopic studies revealed that while aggregation was extensive, most of the aggregate consisted of void volume. Cell growth studies demonstrated that human diploid fibroblasts proliferated well in microcarrier aggregates, reaching densities of 2.5-3×10(6) cells per 2 ml dish after 6 days from an inoculum of 0.5×10(6) cells per dish. When cells were added to the microcarriers at higher density (up to 5×10(6) cells per 2-ml culture), there was little net growth but the cells remained viable over a 7-day period. In contrast, cells died when plated under the same conditions in monolayer culture. When the microcarriers were used in suspension culture, rapid cell attachment and rapid microcarrier aggregation also occurred. In 100-ml suspension culture, a cell density of 0.7×10(6) cells per ml was reached after 7 days from an inoculum of 0.1×10(6) cells. Based on these da...Continue Reading

References

Mar 1, 1991·Applied Microbiology and Biotechnology·S Goetghebeur, W S Hu
Jul 1, 1985·The Journal of Clinical Investigation·S H PhanD Smith

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Citations

Oct 19, 2010·Cytotechnology·Maizirwan MelYusilawati Ahmad Nor
Apr 15, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Dai-Xu WeiGuo-Qiang Chen
Apr 11, 2018·Tissue Engineering. Part B, Reviews·Max J LermanJohn P Fisher
Aug 21, 2010·Chemistry : a European Journal·Gion CalzaferriRobert Häner

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