PMID: 6172777Dec 11, 1981Paper

Human DNA sequences complementary to the small nuclear RNA U2

Nucleic Acids Research
G WestinU Pettersson

Abstract

Clones containing sequences complementary to the small nuclear RNA U2 were isolated from a human DNA library (1). Three clones, designated U2/4, U2/6 and U2/7 were purified and characterized by restriction enzyme cleavage, hybridization and heteroduplex analysis. Hybridization showed that the three clones each contained one single region which is complementary to U2 RNA. Restriction enzyme cleavage revealed furthermore that the inserted fragments in the three recombinants are different. Heteroduplex analysis identified a 240-380 bp long duplex region in each heteroduplex which includes sequences complementary to U2 RNA. Heteroduplexes between clones U2/4 and U2/7 as well as between U2/4 and U2/6 revealed two additional approximately 200 bp long homologies. The remainder of the inserts were found to lack measurable sequence homology. Two fragments from clone U2/4 were subcloned in the pBR322 vector and the subclones were used to determine the nucleotide sequence of a region in clone U2/4 which is complementary to U2 RNA. A comparison between the established sequence and the sequence for rat U2 RNA (2) reveals several discrepancies.

References

Jan 1, 1978·Nucleic Acids Research·B VennströmL Philipson
Dec 14, 1971·Journal of Molecular Biology·R W Davis, R W Hyman
Aug 1, 1969·Biochemistry·B L McConaughyB J McCarthy
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Sep 25, 1980·Nucleic Acids Research·C BranlantP Jeanteur
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R A DenisonA M Weiner
Jul 24, 1981·Nucleic Acids Research·K Hayashi
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·Y OhshimaT Miyata
Apr 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J Rogers, R Wall
Jan 1, 1981·Annual Review of Biochemistry·R Breathnach, P Chambon
Jan 10, 1980·Nature·M R LernerJ A Steitz

❮ Previous
Next ❯

Citations

Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G WestinU Pettersson
Aug 11, 1982·Nucleic Acids Research·M PiechaczykP Jeanteur
Aug 15, 1983·Journal of Molecular Biology·T TaniY Ohshima
Jun 25, 1983·Journal of Molecular Biology·H J MonsteinU Pettersson
Sep 25, 1983·Journal of Molecular Biology·A AlonsoE Spiess
Oct 25, 1984·Journal of Molecular Biology·K HammarströmU Petterson
May 1, 1985·Molecular and Cellular Biology·C BarkU Pettersson
Mar 1, 1984·Molecular and Cellular Biology·S W Van Arsdell, A M Weiner
Aug 1, 1985·Experimental Cell Research·K HammarströmU Pettersson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.