Human eggs, zygotes, and embryos express the receptor angiotensin 1-converting enzyme 2 and transmembrane serine protease 2 protein necessary for severe acute respiratory syndrome coronavirus 2 infection.

F&S Science
Sandeep K RajputRebecca L Krisher

Abstract

To study messenger ribonucleic acid (mRNA) and protein expressions of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) entry receptors (angiotensin 1-converting enzyme 2 [ACE2] and CD147) and proteases (transmembrane serine protease 2 [TMPRSS2] and cathepsin L [CTSL]) in human oocytes, embryos, and cumulus (CCs) and granulosa cells (GCs). Research study. Clinical in vitro fertilization (IVF) treatment center. Patients undergoing IVF were treated at the Colorado Center for Reproductive Medicine. Oocytes (germinal vesicle and metaphase II [MII]) and embryos (1-cell [1C] and blastocyst [BL]) were donated for research at the disposition by the patients undergoing IVF. Follicular cells (CC and GC) were collected from women undergoing egg retrieval after ovarian stimulation without an ovulatory trigger for in vitro maturation/IVF treatment cycles. Presence or absence of ACE2, CD147, TMPRSS2, and CTSL mRNAs detected using quantitative reverse transcription polymerase chain reaction and proteins detected using capillary Western blotting in human oocytes, embryos, and ovarian follicular cells. The quantitative reverse transcription polymerase chain reaction analysis revealed high abundance of ACE2 gene transcripts in germina...Continue Reading

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Citations

Jun 2, 2021·Systems Biology in Reproductive Medicine·George AnifandisIoannis E Messinis

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Methods Mentioned

BETA
biopsy
glycosylation

Software Mentioned

Simple Western Compass
PerlPrimer
Ensemble genome browser
Compass
Jess
Prism

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