Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor

Transplantation Proceedings
Takanori TakebeHideki Taniguchi

Abstract

Transplantation of bioengineered elastic cartilage is considered to be a promising approach for patients with craniofacial defects. We have previously shown that human ear perichondrium harbors a population of cartilage progenitor cells (CPCs). The aim of this study was to examine the use of a rotating wall vessel (RWV) bioreactor for CPCs to engineer 3-D elastic cartilage in vitro. Human CPCs isolated from ear perichondrium were expanded and differentiated into chondrocytes under 2-D culture conditions. Fully differentiated CPCs were seeded into recently developed pC-HAp/ChS (porous material consisted of collagen, hydroxyapatite, and chondroitinsulfate) scaffolds and 3-D cultivated utilizing a RWV bioreactor. 3-D engineered constructs appeared shiny with a yellowish, cartilage-like morphology. The shape of the molded scaffold was maintained after RWV cultivation. Hematoxylin and eosin staining showed engraftment of CPCs inside pC-HAp/ChS. Alcian blue and Elastica Van Gieson staining showed of proteoglycan and elastic fibers, which are unique extracellular matrices of elastic cartilage. Thus, human CPCs formed elastic cartilage-like tissue after 3-D cultivation in a RWV bioreactor. These techniques may assist future efforts to ...Continue Reading

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Citations

Oct 16, 2013·Tissue Engineering. Part a·Renata Giardini-RosaStephen D Waldman
Sep 7, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Leonardo GuastiPatrizia Ferretti
Aug 19, 2015·Journal of Burn Care & Research : Official Publication of the American Burn Association·Kartik IyerJohn E Greenwood
Mar 30, 2018·Stem Cells and Development·Daniela GrimmAlamelu Sundaresan
Jan 31, 2019·The Laryngoscope·Loraine L Y ChiuStephen D Waldman
Mar 10, 2015·The International Journal of Artificial Organs·Weina ShuHong Kang
Sep 15, 2020·Advanced Biosystems·N CastroS Lanceros-Mendez
Nov 15, 2020·International Journal of Molecular Sciences·Masahiro EnomuraHideki Taniguchi
Jan 15, 2019·Acta Biomaterialia·Ashley N LeberfingerIbrahim T Ozbolat
May 1, 2021·International Journal of Molecular Sciences·Jianhua ZhangRalph Müller
Jul 6, 2021·Artificial Organs·Vishal SwaminathanVakhtang Tchantchaleishvili

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