Human embryo-conditioned medium stimulates in vitro endometrial angiogenesis

Fertility and Sterility
Kitty KapiteijnFrans M Helmerhorst

Abstract

Successful implantation and placentation depend on the interaction between the endometrium and the embryo. Angiogenesis is crucial at this time. In this article we investigate the direct influence of the human embryo on in vitro endometrial angiogenesis. In vitro study. Human endometrial microvascular endothelial cells (hEMVEC) grown on an in vitro angiogenesis model. Conditioned media (CM) of human embryos were used to stimulate in vitro angiogenesis. In vitro angiogenesis of hEMVEC. Conditioned media of human embryos, containing significant amounts of vascular endothelial growth factor (VEGF)-A, as determined by enzyme-linked immunosorbent assay (ELISA), caused an increase in hEMVEC tube formation. This effect was prevented by soluble VEGF receptor 1, which quenches VEGF-A activity. Recombinant EGF alone and leukemia inhibitory factor in combination with VEGF-A stimulated hEMVEC tube formation. None of the other tested recombinant mediators, which have been described as produced by the early embryo/trophoblast (interleukin (IL) 10, transforming growth factor (TGF) beta, placental growth factor, hCG, colony-stimulating factor 1, interferon-gamma, insulin-like growth factor I and II, IL-6, platelet-derived growth factor, and TG...Continue Reading

Citations

Jul 10, 2007·Journal of Assisted Reproduction and Genetics·Donald S TorryRonald J Torry
Feb 19, 2016·The Journal of Obstetrics and Gynaecology Research·Peng ChenYan-Ming Liang
May 22, 2008·Reproductive Biomedicine Online·Cammi GoodmanC B Coulam
Apr 13, 2010·Reproductive Biomedicine Online·Els GroeneveldCornelis B Lambalk
Jun 2, 2009·Acta histochemica·Ramazan DemirBerthold Huppertz
Mar 8, 2008·Fertility and Sterility·Kitty KapiteijnFrans M Helmerhorst
Jun 15, 2011·Reproductive Biomedicine Online·Sarah NordqvistHelena Akerud
Dec 15, 2010·Best Practice & Research. Clinical Obstetrics & Gynaecology·Margreet Plaisier
Mar 27, 2007·Fertility and Sterility·Gregory W ThomasDavid Bar-Or
Apr 25, 2017·Journal of Cellular Biochemistry·Mostafa Peyvandi KarizbodaghHamed Mirzaei
Jul 20, 2010·Reproductive Sciences·Sarah NordqvistHelena Akerud
Aug 22, 2008·Immunological Investigations·Caroline DunkRebecca Lee Jones
Aug 17, 2020·The Cochrane Database of Systematic Reviews·Charalampos S SiristatidisDennis Vaidakis

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