Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection.

AIDS Research and Therapy
Sriram Bandi, Ramesh Akkina

Abstract

Human embryonic stem (hES) cells hold considerable promise for cell replacement and gene therapies. Their remarkable properties of pluripotency, self-renewal, and tractability for genetic modification potentially allows for the production of sizeable quantities of therapeutic cells of the hematopoietic lineage. Dendritic cells (DC) arise from CD34+ hematopoietic progenitor cells (HPCs) and are important in many innate and adaptive immune functions. With respect to HIV-1 infection, DCs play an important role in the efficient capture and transfer of the virus to susceptible cells. With an aim of generating DCs from a renewable source for HIV-1 studies, here we evaluated the capacity of hES cell derived CD34+ cells to give rise to DCs which can support HIV-1 infection. Undifferentiated hES cells were cultured on S17 mouse bone marrow stromal cell layers to derive CD34+ HPCs which were subsequently grown in specific cytokine differentiation media to promote the development of DCs. The hES derived DCs (hES-DC) were subjected to phenotypic and functional analyses and compared with DCs derived from fetal liver CD34+ HPC (FL-DC). The mature hES-DCs displayed typical DC morphology consisting of veiled stellate cells. The hES-DCs also di...Continue Reading

References

Mar 1, 1997·Immunology Today·G Girolomoni, P Ricciardi-Castagnoli
Nov 6, 1998·Science·J A ThomsonJ M Jones
Apr 5, 2000·Nature Biotechnology·B E ReubinoffA Bongso
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·J BaiR Akkina
Sep 28, 2000·Current Opinion in Immunology·P J Fairchild, H Waldmann
Jul 27, 2001·Diabetes·S AssadyM Tzukerman
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·D S KaufmanJ A Thomson
Sep 21, 2002·Circulation Research·Chunhui XuMelissa K Carpenter
Nov 18, 2003·Immunological Reviews·Adrian E Morelli, Angus W Thomson
Jun 1, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·David S StrayerJohn A Zaia
Aug 13, 2005·Current Opinion in Biotechnology·Colin H Martin, Dan S Kaufman
Jul 18, 2006·Proceedings of the National Academy of Sciences of the United States of America·Zoran GalicJerome A Zack
Oct 26, 2006·Nature Reviews. Immunology·Li Wu, Vineet N KewalRamani
Apr 5, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joseph AndersonRamesh Akkina
Sep 27, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·David AndersonChris Denning

❮ Previous
Next ❯

Citations

Apr 8, 2009·Pathology Oncology Research : POR·A Saleh YounesGyörgy Berencsi
May 9, 2012·The Journal of Antimicrobial Chemotherapy·Javier A EstévezMarta Valle
Aug 15, 2014·British Journal of Cancer·R J GeraghtyUNKNOWN Cancer Research UK
May 6, 2010·Expert Review of Clinical Immunology·Ashleigh S Boyd, Paul J Fairchild
Sep 24, 2015·Nature Reviews. Drug Discovery·Erin A Kimbrel, Robert Lanza
Jan 21, 2011·Virology·Scott G KitchenDong Sung An
May 7, 2009·Current Opinion in Organ Transplantation·Kathryn M Silk, Paul J Fairchild
Jan 19, 2011·Current Opinion in HIV and AIDS·Scott G Kitchen, Jerome A Zack

❮ Previous
Next ❯

Methods Mentioned

BETA
genetic modification
FACS
fluorescence microscopy
ELISA
scraping

Software Mentioned

EXPO

Related Feeds

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.