PMID: 8608406Oct 1, 1995Paper

Human endometrial carcinoma cells release factors which inhibit the growth of normal epithelial cells in culture

Cell Biology and Toxicology
C D AlbrightD G Kaufman

Abstract

Autocrine and paracrine interactions between cells are important homeostatic mediators in normal tissues. Alterations to growth factor signalling pathways are likely to play a role in multistep carcinogenesis. In this study normal human endometrial epithelial cells (NHEC) after 3 days in culture were treated with serum-free medium conditioned for 24 h by log phase or confluent cultures of established RL95-2, HEC1A, or AN3CA endometrial carcinoma (EC) cell lines. By day 4, NHEC treated with either log phase or confluent conditioned medium (CM) showed a significant decrease (approximately 50-90% of control) in [3H]thymidine ([3H]TdR) incorporation. DNA synthesis was inhibited more by confluent than by log phase CM. By day 7, NHEC treated with CM exhibited fewer colonies per culture, fewer cells per colony, and an increased percentage of single cells. Several growth-regulatory gene products found in the nucleus or at the cell membrane have been shown to be expressed differently in normal and transformed cells. We selected the p53 and c-Ha-ras p21 proteins to further investigate the mechanism of alteration of proliferation in cells treated with carcinoma CM. Thus, by day 7, the percentage of NHEC with nuclear localization of wild t...Continue Reading

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Citations

Apr 28, 2000·Fertility and Sterility·S SawatsriN Sidell
Oct 17, 2012·Experimental and Molecular Pathology·Scott R Schlemmer, David G Kaufman
Mar 27, 2001·Experimental and Molecular Pathology·C D Albright, D G Kaufman
Jan 11, 2002·Experimental and Molecular Pathology·Craig D AlbrightJames H Resau

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