Human Endometrial Exosomes Contain Hormone-Specific Cargo Modulating Trophoblast Adhesive Capacity: Insights into Endometrial-Embryo Interactions

Biology of Reproduction
David W GreeningLois A Salamonsen

Abstract

Embryo implantation into receptive endometrium requires synergistic endometrial-blastocyst interactions within the uterine cavity and is essential for establishing pregnancy. We demonstrate that exosomes (40-150 nm nanovesicles) released from endometrial epithelial cells are an important component of these interactions. We defined the proteome of purified endometrial epithelial-derived exosomes (Exos) influenced by menstrual cycle hormones estrogen (E; proliferative phase) and estrogen plus progesterone (EP; receptive phase) and examined their potential to modify trophoblast function. E-/EP-Exos were uniquely enriched with 254 and 126 proteins, respectively, with 35% newly identified proteins not previously reported in exosome databases. Importantly, EP-Exos protein cargo was related to fundamental changes in implantation: adhesion, migration, invasion, and extracellular matrix remodeling. These findings from hormonally treated ECC1 endometrial cancer cells were validated in human primary uterine epithelial cell-derived exosomes. Functionally, exosomes were internalized by human trophoblast cells and enhanced their adhesive capacity, a response mediated partially through active focal adhesion kinase (FAK) signaling. Thus, exoso...Continue Reading

References

Feb 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S K Hanks, T R Polte
Mar 1, 1997·Human Reproduction Update·M T KaneC Coonan
May 1, 1997·Reviews of Reproduction·J D Aplin
May 5, 1998·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C ColnotF Poirier
May 8, 1998·Human Reproduction·L VićovacM Cuperlović
Jun 11, 1999·The New England Journal of Medicine·A J WilcoxC R Weinberg
Jun 18, 1999·Matrix Biology : Journal of the International Society for Matrix Biology·M Slater, C R Murphy
May 8, 2000·Seminars in Reproductive Endocrinology·M J Murray, B A Lessey
Mar 13, 2002·The Journal of Clinical Endocrinology and Metabolism·Steven L YoungBogdan J Nowicki
May 15, 2002·Reviews in Endocrine & Metabolic Disorders·Bruce A Lessey, Arthur J Castelbaum
Dec 12, 2002·Cells, Tissues, Organs·Jun Wang, D Randall Armant
Jan 18, 2003·Science·Asgerally T Fazleabas, J Julie Kim
Jan 18, 2003·Science·Olga D GenbacevSusan J Fisher
Apr 18, 2003·Frontiers in Bioscience : a Journal and Virtual Library·Steven K HanksJan Brábek
Jun 5, 2003·Nature Reviews. Molecular Cell Biology·Rupert TimplMon-Li Chu
Sep 3, 2004·The Journal of Biological Chemistry·Kiyohito TairaKen-ichi Kariya
Sep 9, 2004·Cell Research·Christopher R Murphy
Jul 13, 2005·Molecular & Cellular Proteomics : MCP·Alexey I Nesvizhskii, Ruedi Aebersold
Dec 31, 2005·Nucleic Acids Research·Frank DesiereRuedi Aebersold
Jul 1, 2006·International Journal of Biological Sciences·Yohei TominagaChu-Xia Deng
Aug 12, 2006·Journal of Molecular Endocrinology·Ying ChenZeng-Ming Yang
Sep 20, 2006·Human Reproduction Update·Hanna Achache, Ariel Revel
Dec 16, 2006·Journal of Cellular Biochemistry·Ying Chen, Xiao-Yan Du
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Nov 23, 2007·Molecular Biology of the Cell·Christian DimaanoMarkus Babst

❮ Previous
Next ❯

Citations

Mar 13, 2016·Birth Defects Research. Part C, Embryo Today : Reviews·Lien M Davidson, Kevin Coward
Jul 23, 2016·Nature Reviews. Endocrinology·Jemma EvansEva Dimitriadis
Dec 14, 2016·Annual Review of Animal Biosciences·Olivier SandraIsabelle Hue
Sep 9, 2016·Scientific Reports·David W GreeningRichard J Simpson
May 12, 2016·Reproduction, Fertility, and Development·Samson N DowlandChristopher R Murphy
Jan 1, 2016·Reproduction, Fertility, and Development·T E SpencerP Lonergan
Jun 21, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·Carmen AlmiñanaPascal Mermillod
Jan 19, 2018·Biology of Reproduction·Gregory W BurnsThomas E Spencer
Nov 9, 2017·Frontiers in Pharmacology·Majid AlhomraniWilliam Sievert
Nov 23, 2017·Reproductive Biology and Endocrinology : RB&E·Jingjie LiangZhengguang Wang
Nov 1, 2017·The Journal of Pathology·Hendrika M DuivenvoordenBelinda S Parker
Jul 24, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jemma EvansLois A Salamonsen
Oct 27, 2018·Journal of Assisted Reproduction and Genetics·Noble K Kurian, Deepak Modi
Feb 23, 2018·Nature·George C PattonSusan M Sawyer
Feb 2, 2018·Endocrine Reviews·Carlos SimonFelipe Vilella
Apr 17, 2019·Journal of Cellular Physiology·Ali RanjbaranNathalie Raffel
Aug 10, 2019·International Journal of Molecular Sciences·Qingqing LiuJianping Chen
Nov 23, 2019·Cells·Valentina Karin-KujundzicLjiljana Serman
Nov 22, 2019·Current Drug Metabolism·Xiaoyan SunXuehong Zhang
Mar 17, 2020·Journal of Cellular and Molecular Medicine·Jiayin ZhangXu Zhang
Dec 13, 2019·Stem Cells·Yun Ha HurMarc A Antonyak
Mar 25, 2020·Current Opinion in Obstetrics & Gynecology·Elisa GiacominiPaola Viganó
Oct 18, 2018·Expert Review of Proteomics·David W Greening, Richard J Simpson
Feb 14, 2020·International Journal of Molecular Sciences·Alessandra BridiJuliano Coelho da Silveira
May 30, 2020·International Journal of Molecular Sciences·Marcia de Almeida Monteiro Melo FerrazNucharin Songsasen
Jul 31, 2020·International Journal of Molecular Sciences·Charles BanliatMarie Saint-Dizier
Jun 15, 2018·Biology of Reproduction·Vinay ShuklaAnila Dwivedi
Feb 20, 2019·Journal of Assisted Reproduction and Genetics·David F Albertini
May 6, 2019·International Journal of Molecular Sciences·Francesca AndronicoCinzia Di Pietro
Jun 14, 2019·Molecular Reproduction and Development·Gabriella Mamede AndradeJuliano Coelho da Silveira

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.