Human endothelial and foetal femur-derived stem cell co-cultures modulate osteogenesis and angiogenesis

Stem Cell Research & Therapy
Stefanie InglisRichard O C Oreffo

Abstract

A dynamic vasculature is a prerequisite for bone formation where the interaction of bone cells and endothelial cells is essential for both the development and the healing process of bone. Enhanced understanding of the specific mediators involved in bone cell and endothelial cell interactions offers new avenues for skeletal regenerative applications. This study has investigated the osteogenic and angiogenic potential of co-cultures of human foetal diaphyseal or epiphyseal cells with human umbilical vein endothelial cells (HUVEC) in the presence and absence of vascular endothelial growth factor (VEGF) supplementation. Early osteogenic activities of the co-cultures (± VEGF) were assessed by alkaline phosphatase (ALP) activity. Osteogenic and angiogenic gene expression was measured using quantitative polymerase chain reaction. An ex vivo organotypic embryonic chick (E11) femur culture model was used to determine the osteogenic effects of VEGF as determined using micro-computed tomography (μCT) and Alcian blue/Sirius red histochemistry and immunocytochemistry for expression of CD31. ALP activity and gene expression of ALP and Type-1 collagen was enhanced in foetal skeletal/HUVECs co-cultures. In foetal diaphyseal/HUVECs co-cultures,...Continue Reading

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Citations

Nov 8, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Stefanie InglisRichard O C Oreffo
May 12, 2019·Journal of Biomedical Materials Research. Part a·Amin RahmaniForough Azam Sayahpour
Oct 26, 2018·Stem Cell Research & Therapy·Yang ZhangJeroen J J P van den Beucken
Nov 13, 2018·Bulletin of Experimental Biology and Medicine·I N SaburinaV S Repin
Nov 30, 2019·Scientific Reports·Robin M H RumneyRichard O C Oreffo
Jun 1, 2021·Frontiers in Bioengineering and Biotechnology·Earnest P ChenJohn P Geibel
Nov 12, 2018·ACS Biomaterials Science & Engineering·Carley OrtKibret Mequanint
Apr 27, 2021·Frontiers in Cell and Developmental Biology·Anastasiya A GorkunIrina N Saburina

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Methods Mentioned

BETA
FCS
PCR
fluorescence-activated cell sorting

Software Mentioned

NRecon
OlyVIA
GraphPad Prism
AB7500 SDS
CT Analyser

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