PMID: 6971878May 1, 1981Paper

Human endothelial cell-lymphocyte interaction. Endothelial cells function as accessory cells necessary for mitogen-induced human T lymphocyte activation in vitro

The Journal of Clinical Investigation
E R AshidaP E Lipsky

Abstract

Mitogen-stimulated human T cell activation is absolutely dependent on the participation of a nonresponding accessory cell. In populations of human peripheral blood mononuclear cells, monocytes function as the requisite accessory cells. The possibility that cultured endothelial cells (EC) might also function as accessory cells was studied by examining the potential of endothelial cells to restore mitogen responsiveness to monocyte-depleted human T cells. Highly purified T cells were prepared by isolating cells rosetting with sheep erythrocytes and removing monocyte contamination by glass adherence and nylon wool column passage. When cultured at low cell density, T cells failed to respond to stimulation with various mitogenic lectins, whereas co-culture with monocytes restored responsiveness. Similarly, EC obtained from umbilical vein, pulmonary artery, and ovarian vein restored the capacity of T cells to respond to mitogens. Mitogen-stimulated T cell activation required viable endothelial cells. Moreover, effective endothelial T cell cooperation appeared to involve the establishment of cell-to-cell contact between EC and responding T cells. Accessory cell function was not a nonspecific property of all tissue culture cells as evi...Continue Reading

References

Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R M Steinman, M D Witmer
Jun 1, 1975·Transplantation·H HirschbergE Thorsby
Jul 1, 1977·European Journal of Immunology·S Habu, M C Raff
Dec 1, 1979·Cellular Immunology·A L MaizelR J Ford
Mar 1, 1977·The Journal of Clinical Investigation·P E Lipsky, M Ziff
Aug 1, 1977·The Journal of Clinical Investigation·J R Moraes, P Stastny
Jun 21, 1974·Science·H A Crissman, R A Tobey
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·V UgoliniJ D Capra
Jan 1, 1980·Scandinavian Journal of Immunology·P HäyryL C Andersson

❮ Previous
Next ❯

Citations

Feb 1, 1990·Cancer Metastasis Reviews·P N Belloni, R J Tressler
Jan 1, 1994·Rheumatology International·R SauraK Mizuno
Apr 1, 1983·Journal of Clinical Immunology·C W Spellman, T B Tomasi
Jan 1, 1991·Pharmacology & Therapeutics·R Eisenstein
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J S Pober, M A Gimbrone
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D D HoM S Hirsch
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·E R Ashida, V L Scofield
Apr 1, 1983·The Journal of Experimental Medicine·J S PoberK A Ault
Nov 19, 2004·Transplantation·Franck JoucherMichele German-Fattal
Apr 1, 1992·Scandinavian Journal of Immunology·J R WestphalR M De Waal
Nov 1, 1991·Annals of the Rheumatic Diseases·A KaulJ D Pearson
May 1, 1987·The Journal of Clinical Investigation·T Matsubara, M Ziff
Apr 29, 2004·BMC Immunology·Annette L RothermelDavid R Johnson
Jan 1, 1989·Archives of Dermatological Research·M FimianiL Andreassi
Aug 1, 1993·In Vitro Cellular & Developmental Biology. Animal·K Spanel-Borowski, G Bein
Jul 1, 1984·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·R G ShawB Combes
Jul 1, 1995·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·J K DesaiP R Bell
Feb 9, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·S S WalusimbiJ L Pate
Jun 30, 1988·Biochemical and Biophysical Research Communications·K NoriokaH Nakamura
Feb 1, 1982·Immunology Today·P E Lipsky, J R Kettman
Nov 1, 1989·Clinical Immunology and Immunopathology·T KashiwadoM Ziff
Jan 1, 1984·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·G FryR Czervionke
Feb 1, 1989·Scandinavian Journal of Immunology·J M TeitelA Schiavone
Sep 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J Y Scoazec, G Feldmann
Aug 11, 2000·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·J P Chen, T M Cosgriff
Nov 1, 1986·Current Eye Research·M K KimC C Chan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.