PMID: 8581846Nov 1, 1995Paper

Human eosinophils preferentially survive on tissue fibronectin compared with plasma fibronectin

Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology
G M WalshA J Wardlaw

Abstract

Eosinophil-derived inflammatory mediators including cytokines are considered to be important in the pathogenesis of allergic inflammation. Fibronectin (Fn) has been shown to be a physiological trigger of autocrine cytokine production by human eosinophils. Fn is encoded by a single gene, but alternate splicing of the primary RNA transcript results in polypeptide diversity in a cell type-specific fashion. Thus, tissue Fn contains approximately 50% more of the CS-1 cell binding region recognized by the integrin alpha 4 beta 1 compared with plasma Fn. Since eosinophils are predominantly tissue-dwelling cells we compared the effect of tissue and plasma Fn on eosinophil survival in culture. The viability and cytokine generation of eosinophils (> 99.9% pure) cultured for up to 4 days in 96 well plates coated with tissue Fn, plasma Fn or BSA was compared. There was a significant difference in the ability of tissue Fn to support eosinophil survival compared with plasma Fn (P < 0.01). Optimal survival with tissue Fn was seen at 25 micrograms/well (70% +/- 2.0% viability at 3 days vs 7% +/- 2.2% viability on BSA). Significant (P < 0.001) cell viability on tissue Fn was observed for up to 4 days in culture (54% +/- 6.0%) compared with BSA ...Continue Reading

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Citations

Aug 14, 1999·Scandinavian Journal of Immunology·A MoshfeghJ Lundahl
Feb 2, 1999·American Journal of Respiratory and Critical Care Medicine·J MeerschaertN N Jarjour
Jun 14, 2000·American Journal of Respiratory and Critical Care Medicine·E LeeS Kilfeather
Feb 26, 1998·American Journal of Respiratory Cell and Molecular Biology·K A PachecoL J Rosenwasser
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·G DewsonA J Wardlaw
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·H YamamotoW W Busse
Jan 1, 1997·Memórias do Instituto Oswaldo Cruz·M C Seminario, B S Bochner
Nov 24, 1999·Critical Reviews in Clinical Laboratory Sciences·G M Walsh
Jun 10, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A J Wardlaw
Jan 1, 2013·Journal of Cell Death·Garry M Walsh
May 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·G M Walsh
Aug 2, 2001·Archives of Environmental Health·A J ThompsonC C Patterson
Jan 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne Burke-GaffneyPaul G Hellewell
Nov 9, 2000·American Journal of Rhinology·C BachertP van Cauwenberge

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