Human Epicardial Fat Expresses Glucagon-Like Peptide 1 and 2 Receptors Genes

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme
Gianluca IacobellisGaofeng Wang

Abstract

Epicardial adipose tissue (EAT) is an easily measurable visceral fat of the heart with unique anatomy, functionality, and transcriptome. EAT can serve as a therapeutic target for pharmaceutical agents targeting the fat. Glucagon-like peptide-1 (GLP-1) and GLP-2 analogues are newer drugs showing beneficial cardiovascular and metabolic effects. Whether EAT expresses GLP- 1 and 2 receptors (GLP-1R and GLP-2R) is unknown. RNA-seq analysis and quantitative real-time polymerase chain reaction (qRT-PCR) were performed to evaluate the presence of GLP-1R and GLP-2R in EAT and subcutaneous fat (SAT) obtained from 8 subjects with coronary artery disease and type 2 diabetes mellitus undergoing elective cardiac surgery. Immunofluorescence was also performed on EAT and SAT samples using Mab3f52 against GLP-1R. Our RNA-sequencing (RNA-seq) analysis showed that EAT expresses both GLP-1R and GLP-2R genes. qRT-PCR analysis confirmed that GLP-1R expression was low but detected by 2 different sets of intron-spanning primers. GLP-2R expression was detected in all patients and was found to be 5-fold higher than GLP-1R. The combination of accurately spliced reads from RNA-seq and successful amplification using intron-spanning primers indicates that b...Continue Reading

Citations

Mar 20, 2018·Endocrinology·Gianluca Iacobellis
Sep 27, 2018·World Journal of Diabetes·Eleni XourgiaAndreas Melidonis
Mar 20, 2020·Journal of the Endocrine Society·Gianluca Iacobellis, Alexandra C Villasante Fricke
Mar 17, 2020·Endocrine·Sara BaldassanoFlavia Mulè
Sep 29, 2020·British Journal of Pharmacology·Miriam EjarqueSonia Fernández-Veledo
Dec 16, 2020·Endocrine Reviews·Brent A McLeanDaniel J Drucker
Jan 15, 2021·Molecular and Cellular Endocrinology·Jorge Felipe Argenta ModelAnapaula Sommer Vinagre
Nov 27, 2019·Molecular Metabolism·T D MüllerM H Tschöp
Apr 13, 2021·Journal of Cardiovascular Pharmacology and Therapeutics·Emir M MuzurovićDimitri P Mikhailidis
May 15, 2021·Heart·Sarah L AytonAlastair J Moss
Aug 6, 2020·Archives of Cardiovascular Diseases·Valentina MangiaficoSyed Ahsan
Oct 14, 2021·Journal of Endocrinological Investigation·G Iacobellis, M G Baroni

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