Human epidermal basal keratinocytes express CDw29 antigens.

The British Journal of Dermatology
M J StaquetD Schmitt

Abstract

Two monoclonal antibodies, K20 and 4B4, assigned to the CDw29 cluster of differentiation antigens, were shown to react with basal keratinocytes (BK). The aim of this study was to identify the antigens recognized by K20 and 4B4 on epidermal cells, and to determine whether they were identical to those found on lymphocytes. Basal keratinocyte-enriched cell suspensions were labelled with 125I and then 1% NP40 cell lysates were used for immunoprecipitation. Under reducing conditions, K20 and 4B4 immunoprecipitated from basal keratinocytes a broad MW 105,000 band and proteins of MW 145,000, 90,000 and 80,000. Under non-reducing conditions, each band was shifted down by approximately 5000 MW. Metabolic labelling studies demonstrated that the MW 145,000 and 105,000 subunits were synthesized by basal keratinocytes. On lymphoid cells, K20 and 4B4 are known to precipitate glycoprotein complexes made of a broad MW 130,000 protein band (beta subunit) associated with a protein of MW 150,000 (alpha subunit) and proteins of MW 90,000 and 80,000 expressed in very low amounts. The MW 145,000 and 105,000 bands immunoprecipitated by K20 from basal keratinocytes correspond to the alpha and beta subunits present on lymphoid cells. It has recently be...Continue Reading

References

Feb 1, 1987·The Journal of Dermatology·M J StaquetJ Thivolet
Apr 1, 1988·Immunology Today·M E Hemler
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K H SingerB F Haynes
May 1, 1987·The Journal of Investigative Dermatology·J D BosM L Kapsenberg
Jul 1, 1985·The Journal of Investigative Dermatology·K H SingerB F Haynes
Jan 1, 1980·Immunological Reviews·G StinglS I Katz
Sep 1, 1983·The Journal of Investigative Dermatology·A C ChuR L Edelson
Jun 1, 1983·The Journal of Investigative Dermatology·J W Streilein
Jul 1, 1982·The Journal of Investigative Dermatology·D N SauderJ J Oppenheim
Jan 1, 1980·The Journal of Investigative Dermatology·J R StanleyS I Katz

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Citations

Jun 21, 2002·Acta Microbiologica Et Immunologica Hungarica·Lívia BerekJ Téren
May 18, 2016·The Journal of Investigative Dermatology·Ester Ruiz-RomeuLuis F Santamaria-Babí
Apr 1, 1990·Experimental Cell Research·M J StaquetJ Thivolet
Apr 1, 1991·Clinical and Experimental Immunology·C HorrocksA W Thomson

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