PMID: 6538880Apr 1, 1984Paper

Human epithelial cell intermediate filaments: isolation, purification, and characterization

The Journal of Cell Biology
M W AynardiR D Goldman

Abstract

Intermediate filaments (IF) isolated from human epithelial cells (HeLa) can be disassembled in 8 M urea and reassembled in phosphate-buffered solutions containing greater than 0.1 mg/ml IF protein. Eight proteins were associated with HeLa IF after several disassembly-reassembly cycles as determined by sodium dodecyl sulfate gel electrophoresis (SDS PAGE). A rabbit antiserum directed against HeLa IF contained antibodies to most of these proteins. The immunofluorescence pattern that was seen in HeLa cells with this antiserum is complex. It consisted of a juxtanuclear accumulation of IF protein and a weblike array of cytoplasmic fibers extending to the cell border. Following preadsorption with individual HeLa IF proteins, the immunofluorescence pattern in HeLa cells was altered to suggest the presence of at least two distinct IF networks. The amino acid composition and alpha-helix content (approximately 38%) of HeLa IF proteins was similar to the values obtained for other IF proteins. One-dimensional peptide maps show extensive homology between the major HeLa IF protein of 55,000-mol-wt and a similar 55,000-mol-wt protein obtained from hamster fibroblasts (BHK-21). HeLa 55,000-mol-wt homopolymer IF assembled under conditions simil...Continue Reading

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Citations

Jan 1, 1990·Cell Motility and the Cytoskeleton·K D Brown, L I Binder
Dec 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C L BunnL W Knapp
Jun 16, 2001·Neurochemistry International·G Benítez-KingG Ramírez
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·A E GoldmanR D Goldman
Jul 1, 1984·The Journal of Cell Biology·P M SteinertR D Goldman
Nov 29, 2011·Journal of Structural Biology·Robert D GoldmanEdward R Kuczmarski
Jun 22, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hideki KanayaHideki Sumimoto
Jan 1, 1986·Cell Motility and the Cytoskeleton·K J Green, R D Goldman
Jan 1, 1988·Cell Motility and the Cytoskeleton·L I Rebhun, R E Palazzo
Jan 1, 1989·Cell Motility and the Cytoskeleton·W Steffen, R W Linck
Jul 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D EscalierJ Schrével
Mar 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H KawaharaS W French
Jun 13, 2014·Nature Reviews. Cancer·Louis Vermeulen, Hugo J Snippert
Nov 1, 1985·The Journal of Urology·A A MolinoloA I Sens

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