Human factor D of the alternative pathway: purification and quantitation by enzyme amplified electroimmunoassay

Journal of Immunological Methods
L Truedsson, G Sturfelt

Abstract

Purified factor D was prepared with a yield of about 30%. Monospecific antiserum was raised in rabbits. Immunochemical quantitation of factor D in serum and plasma was performed by electroimmunoassay and a sensitive staining technique based on enzyme amplification. Peroxidase-labeled swine antibodies to rabbit immunoglobulin were applied to the gel after electrophoresis. Immune precipitates were then visualized by staining for peroxidase activity. Factor D could be detected at 50 micrograms/l. The normal concentration of D in serum was 1.6 mg/l, as assessed by this assay.

References

Dec 1, 1978·The Journal of Experimental Medicine·P H Lesavre, H J Müller-Eberhard
Nov 1, 1979·Scandinavian Journal of Clinical and Laboratory Investigation·H Löfberg, A O Grubb
Jan 1, 1978·Journal of Immunological Methods·T KonnoH Hirai
Apr 1, 1972·The Journal of Experimental Medicine·H J Müller-Eberhard, O Götze
Jan 1, 1972·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·C B Laurell
Jan 1, 1973·Scandinavian Journal of Immunology. Supplement·B Weeke
Feb 1, 1974·The Journal of Experimental Medicine·D T FearonS Ruddy
Feb 1, 1965·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R C GRAHAMM J KARNOVSKY

❮ Previous
Next ❯

Citations

Feb 1, 1987·Immunology Letters·A B LaurellA G Sjöholm
Dec 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A G SjöholmC Söderström
Apr 1, 1986·The Journal of Clinical Investigation·P W SandersJ H Galla
Dec 1, 1989·The Journal of Clinical Investigation·P S HiemstraM R Daha
Aug 1, 1993·Clinical and Experimental Immunology·G SturfeltA G Sjöholm

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.