PMID: 6414079Jan 1, 1983Paper

Human fibroblasts, a convenient nuclear substrate for detection of anti-nuclear antibodies including anti-centromere antibodies

Scandinavian Journal of Rheumatology
C G KallenbergT H The

Abstract

Human fibroblast monolayers were applied as a nuclear substrate in indirect immunofluorescence for the detection of anti-nuclear antibodies (ANA). Comparison of the results obtained with this substrate and with conventional rat liver sections led to the conclusion that the application of human fibroblast monolayers as a substrate has the following advantages: 1) better recognition of the distinct nuclear staining patterns; 2) more convenient serum titrations, since a cryostat is not needed and twelve tests can be performed on one slide; 3) detection of significantly higher ANA titres in sera from patients with SLE and MCTD; 4) recognition of a type of ANA that was not detected on rat liver sections. These latter antibodies produced a discrete speckled pattern of fluorescence, which was completely lost after acid elution of the fibroblasts. Using HEp-2 cells in metaphase, it was shown that the antibodies were directed against the centromere regions of the chromosomes. These anti-centromere antibodies were detected in sera from patients with severe Raynaud's phenomenon. Underlying disorders were scleroderma (8 patients, 5 of them with CREST syndrome), Sjögren's syndrome (2 patients), and Raynaud's phenomenon in combination with a...Continue Reading

References

Jan 1, 1978·The American Journal of Medicine·W J Fessel
Jan 1, 1974·American Journal of Clinical Pathology·M HusainF Lucas
Oct 1, 1974·Rinsho byori. The Japanese journal of clinical pathology·H Suzuki
Feb 13, 1967·JAMA : the Journal of the American Medical Association·R J CammarataR H Fennell
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y MoroiE M Tan
Oct 1, 1980·The American Journal of Medicine·M Fritzler, T D Kinsella
Nov 1, 1980·The American Journal of Medicine·C G KallenbergT H The
Sep 28, 1957·British Medical Journal·E J HOLBOROWG D JOHNSON

Citations

Jun 1, 1987·Clinical Rheumatology·C G Kallenberg, P C Limburg
Mar 1, 1990·Clinical Rheumatology·C G Kallenberg
Jan 1, 1985·Scandinavian Journal of Rheumatology·C G KallenbergT H The
Jan 1, 1987·Scandinavian Journal of Rheumatology. Supplement·A I SperanskyT D Bulanova
Mar 7, 2021·Antibodies·Irina A PashninaValeriy A Chereshnev

Related Concepts

Fluorescent Antinuclear Antibodies
Centromere
Chromosomes
Collagen Diseases
Fibroblasts
Immunofluorescence Assay
Immunodiffusion Measurement
Liver
Lupus Erythematosus, Systemic
Mixed Connective Tissue Disease

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