PMID: 7546910Jul 1, 1995Paper

Human foamy virus DNA forms and expression in persistently infected Dami megakaryocytic cells

AIDS Research and Human Retroviruses
J Wybier-FranquiR Emanoil-Ravier

Abstract

We have characterized human foamy virus (HFV) proviral DNA and determined HFV expression in a persistent infection model, the Dami megakaryocytic cell line. Molecular studies were performed on parental persistently infected cells (Dami-P), as well as on derived clones (Dami-Cl). We report that in these nonlytic and non-HFV producer cells, viral DNA was found to be integrated into the cellular genome and that the few free proviral forms detected in Dami-P cells were deleted in their 5' LTR. Our molecular analysis indicates the presence of undeleted 5' LTR forms in the integrated provirus within a proviral population mainly composed of deleted forms. In addition, the deletion in the bel1 trans-activator gene, previously described by Saïb et al., was found to be highly predominant. However, in 5-iodo-2'-deoxyuridine treated Dami-Cl cultures, virus production occurred, providing evidence for the presence of complete viral genome. Analysis of HFV expression in Dami-Cl cells, by Northern blot and immunoprecipitation, shows that the most striking difference between cytolytic and persistent HFV infection was the lack of expression of structural viral proteins, in contrast with Bet protein expression, which is maintained. Our data sugge...Continue Reading

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Citations

Oct 10, 1996·AIDS Research and Human Retroviruses·M Santillana-HayatJ M Molina
Jan 1, 1996·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·A Saïb, H de Thé
Jan 9, 2001·Clinical Microbiology Reviews·C D Meiering, M L Linial
Jun 20, 2001·Journal of Virology·C D MeieringM L Linial

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