Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality

Clinical Proteomics
Xin LiuFujun Liu

Abstract

Human follicular fluid (HFF), which is composed by essential proteins required for the follicle development, provides an important microenvironment for oocyte maturation. Recently, overweight status has been considered as a detrimental impact factor on oocyte maturation, but whether HFF proteome could provide protein markers for assessing overweight-based oocyte maturation deficiency is still unknown. To reveal the HFF-based molecular characteristics associated with abnormal oocyte maturation, an iTRAQ-based comparative proteomic analysis was performed to investigate different HFF protein expression profiles from normal weight women and overweight status women. Two hundred HFF proteins were quantified in our data, of which 43% have not been overlapped by two previous publications. Compared with the HFF proteins of normal weight women, 22 up-regulated HFF proteins and 21 down-regulated HFF proteins were found in the overweight status women. PANTHER database showed these altered HFF proteins participated in development, metabolism, immunity, and coagulation, and STRING database demonstrated their complicated interaction networks. The confidence of proteomic outcome was verified by Western blot analysis of WAP four-disulfide core ...Continue Reading

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Citations

Jan 10, 2019·Cell Death & Disease·Joseph GeorgeMikihiro Tsutsumi

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Methods Mentioned

BETA
electrophoresis
ELISA

Software Mentioned

STRING
Protein pilot
[UNK]

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