Human fructosamine-3-kinase: purification, sequencing, substrate specificity, and evidence of activity in vivo

B S SzwergoldPaul J Beisswenger


Nonenzymatic glycation appears to be an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. In this report, we describe the purification and characterization of a mammalian fructosamine-3-kinase (FN3K), which phosphorylates fructoselysine (FL) residues on glycated proteins, to FL-3-phosphate (FL3P). This phosphorylation destabilizes the FL adduct and leads to its spontaneous decomposition, thereby reversing the nonenzymatic glycation process at an early stage. FN3K was purified to homogeneity from human erythrocytes and sequenced by means of electrospray tandem mass spectrometry. The protein thus identified is a 35-kDa monomer that appears to be expressed in all mammalian tissues. It has no significant homology to other known proteins and appears to be encoded by genomic sequences located on human chromosomes 1 and 17. The lability of FL3P, the high affinity of FN3K for FL, and the wide distribution of FN3K suggest that the function of this enzyme is deglycation of nonenzymatically glycated proteins. Because the condensation of glucose and lysine residues is an ubiquitous and unavoidable process in homeothermic organism...Continue Reading


Oct 1, 1992·Kidney International·M SabbatiniV E Andreucci
Jan 26, 1990·Science·B S SzwergoldT R Brown
Jan 1, 1989·Experimental Cell Research·S TsvetkovL Djondjurov
Jan 1, 1984·Methods in Enzymology·J W BaynesM H Murtiashaw
Aug 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·H B Mortensen, M O Marshal
Feb 9, 1984·The New England Journal of Medicine·D M NathanJ D Goodson
Apr 1, 1995·Archives of Biochemistry and Biophysics·S LalT R Brown
May 25, 1995·Biochemical and Biophysical Research Communications·E FujiiK Hotta
Aug 11, 1993·Molecular and Cellular Biochemistry·F N Ziyadeh, M P Cohen
Aug 5, 1996·Biochemical and Biophysical Research Communications·A OkadoN Taniguchi
Jun 15, 1997·Archives of Biochemistry and Biophysics·S LalT R Brown
Jul 11, 1997·The Journal of Biological Chemistry·J R RequenaS R Thorpe
Jul 30, 1997·Biochemical and Biophysical Research Communications·C HirataM Kondo
Dec 4, 1998·Diabetes·U J ErikssonP J Thornalley
Aug 6, 1999·Current Eye Research·S Swamy-MruthintiE C Abraham

❮ Previous
Next ❯


Oct 31, 2006·Analytical and Bioanalytical Chemistry·René KrauseThomas Henle
Feb 25, 2011·Applied Microbiology and Biotechnology·Veronika Maria DeppeFriedhelm Meinhardt
Sep 1, 2005·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Paul J Thornalley
Oct 22, 2010·Amino Acids·Ram H NagarajAlan W Stitt
Oct 19, 2012·Biotechnology Letters·Katsuhiro KojimaKoji Sode
Jun 2, 2012·Journal of the American Chemical Society·Wenhui Zhang, Anthony S Serianni
Dec 19, 2003·Advances in Renal Replacement Therapy·Thomas Henle, Toshio Miyata
Jun 11, 2008·Current Opinion in Clinical Nutrition and Metabolic Care·Robert M Cohen, Eric P Smith
Oct 7, 2004·Applied and Environmental Microbiology·Shin-Ichi AkazawaYoshiki Tani
Feb 4, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Lorena MoscaAndrea Mosca
Feb 14, 2013·Heart Failure Reviews·Vijaya Lakshmi BodigaSreedhar Bodiga
May 23, 2014·Journal of the American Veterinary Medical Association·Kimberly M Pattullo, Beverly A Kidney
Oct 23, 2010·Amino Acids·Emile Van SchaftingenMaria Veiga-da-Cunha
Mar 13, 2008·Oncology·Maria Gabriella CarusoGiovanni Misciagna
Oct 31, 2012·Molecular Aspects of Medicine·Niki ChondrogianniEfstathios S Gonos
Mar 30, 2010·Revista española de geriatría y gerontología·Alba NaudíReinald Pamplona
Aug 10, 2007·Biochemical and Biophysical Research Communications·Benjamin SzwergoldKerry Loomes
Mar 6, 2007·Advances in Enzyme Regulation·Emile Van SchaftingenMaria Veiga-da-Cunha
Jul 23, 2005·Annals of the New York Academy of Sciences·Sarah M BrownJohn W Baynes
Jul 23, 2005·Annals of the New York Academy of Sciences·James R ConnerBenjamin S Szwergold
Jul 23, 2005·Annals of the New York Academy of Sciences·Benjamin S SzwergoldPaul J Beisswenger
Jun 24, 2015·Biochemical and Biophysical Research Communications·Mouadh MihoubGilbert Richarme
May 27, 2010·Diabetes/metabolism Research and Reviews·Juliette FortpiedEmile Van Schaftingen
Feb 27, 2010·The Journal of Pathology·Hugo Vicente Miranda, Tiago Fleming Outeiro
Jul 22, 2014·Angewandte Chemie·Michael Hellwig, Thomas Henle
Mar 25, 2015·Journal of Mass Spectrometry : JMS·Rico SchmidtAndrej Frolov

❮ Previous
Next ❯

Related Concepts

Related Feeds

American Diabetes Association Journals

Discover the latest diabetes research published by the journals from the American Diabetes Association.