Human Fusobacterium necrophorum strains have a leukotoxin gene and exhibit leukotoxic activity

Journal of Medical Microbiology
Sambasivarao TadepalliSanjeev K Narayanan

Abstract

Fusobacterium necrophorum, a Gram-negative anaerobe, causes a variety of necrotic infections in humans and animals. There are two subspecies: subsp. necrophorum and subsp. funduliforme. In cattle, subsp. necrophorum is more prevalent and production of leukotoxin is a major virulence factor. The leukotoxin operon (lktBAC) consists of three genes, lktB, lktA and lktC, of which lktA is the structural toxin gene. The subspecies identity of human F. necrophorum is less certain and it is not known whether human strains possess the leukotoxin gene or leukotoxin activity. Therefore, the objective of this study was to identify the subspecies status of four human clinical strains of F. necrophorum and determine whether they have the leukotoxin gene or leukotoxin activity. Phenotypic and genotypic characteristics suggested that the four strains belonged to subsp. funduliforme, which was confirmed by sequencing the 16S rRNA gene. Analysis of the four strains by PCR revealed the presence of the leukotoxin operon. Partial DNA sequencing identified one human strain with full-length lktA, whereas the others exhibited considerable heterogeneity in size. All strains had a leukotoxin operon promoter-containing intergenic region similar to that of...Continue Reading

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Citations

Jul 28, 2012·Journal of Oral and Maxillofacial Surgery : Official Journal of the American Association of Oral and Maxillofacial Surgeons·Matthew MurrayJeff Roberts
Dec 1, 2009·American Journal of Otolaryngology·James M RidgwayBrian J-F Wong
May 29, 2013·Journal of Basic Microbiology·Amit KumarSanjeev Narayanan
Jun 20, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Amit KumarSanjeev K Narayanan
Aug 21, 2010·Clinical Pediatrics·Brian J Young, Russell W Steele
Aug 21, 2010·Clinical Pediatrics·Brian J Young, Russell W Steele
Nov 6, 2014·MBio·Abigail Manson McGuireAshlee M Earl
Feb 24, 2011·Expert Review of Anti-infective Therapy·Gunnar Dahlén, Anders Ebenfelt
Nov 7, 2018·Microbiome·Abigail J S ArmstrongCatherine A Lozupone

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