PMID: 6167620Sep 1, 1981Paper

Human gamma interferon production by leukocytes induced with monoclonal antibodies recognizing T cells

The Journal of Immunology : Official Journal of the American Association of Immunologists
P von WussowW E Stewart

Abstract

The murine monoclonal antibody OKT3 (IgG2), which recognizes the majority of human E-rosette-forming cells, was found to induce interferon (IFN) production in human peripheral lymphocytes in vitro. This IFN was classified as IFN-gamma by virtue of its acid stability, nonneutralization by antisera recognizing human IFN-alpha and beta, and non-cross-reactivity on bovine kidney cells. OKT3 elicited considerable IFN-gamma yields at a concentration of 1 ng/ml, at which only very low mitogenesis of the lymphocytes measured by 3H-thymidine incorporation was seen. The IFN-gamma activity appeared beginning 3 to 6 hr after stimulation and reached its maximum after 36 hr. Anti-human Lyt3, anti-human Lyt2, or anti-human Lyt1 monoclonal antibodies, recognizing all or the majority of the E-rosette-forming cells, did not elicit IFN-gamma production. The OKT4 monoclonal antibody, which defines a subpopulation of T cells containing cells with helper activity, induced marginal levels of IFN-gamma. In contrast, OKT8 monoclonal antibody (defining cytotoxic/suppressor cells) as well as monoclonal or heterologous antibodies recognizing histocompatibility antigens or surface immunoglobulin failed to induce IFN-gamma production. Heterologous anti-thym...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.