Human gammadelta T cell recognition of lipid A is predominately presented by CD1b or CD1c on dendritic cells.

Biology Direct
Yongchun CuiWei He

Abstract

The gammadelta T cells serve as early immune defense against certain encountered microbes. Only a few gammadelta T cell-recognized ligands from microbial antigens have been identified so far and the mechanisms by which gammadelta T cells recognize these ligands remain unknown. Here we explored the mechanism of interaction of human gammadelta T cells in peripheral blood with Lipid A (LA). First, resting gammadelta T cells (mainly Vdelta2 T cells) displayed a strong proliferative response to LA-pulsed monocyte-derived dendritic cells (moDC) and LA-pulsed paraformaldehyde-fixed moDC, but not to free LA in a TCR gammadelta-dependent manner. Second, anti-CD1b or anti-CD1c antibodies could block proliferative response of resting gammadelta T cells to LA-loaded moDC. Besides, only LA-loaded CD1b/CD1c-transfected C1R lymphoblastoma cells (CD1b-/CD1c-C1R) were able to stimulate the proliferation of human gammadelta T cells. Third, the expressions of both Toll-like receptor (TLR)2 and TLR4 on surface of LA-activated gammadelta T cells were upregulated, whereas only anti-TLR4 antibody could partially block their response to LA; Finally LA-loaded moDCs induce gammadelta T cells to produce Th1 cytokines, such as IFN-gamma. Taken together, w...Continue Reading

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Methods Mentioned

BETA
flow cytometry
FCS

Software Mentioned

BD

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