Human gastrointestinal conditions affect in vitro digestibility of peanut and bread proteins.

Food & Function
Amelia Torcello-GómezAlan Mackie

Abstract

As plant proteins are increasingly used as a source of amino acids in the diet, studies on in vitro digestion of plant proteins are key to understand the different factors affecting proteolysis, with the ultimate goal of optimising the nutritional composition/intake of plant protein-rich products. More realistic scenarios including the most likely food matrix and physiologically relevant gastrointestinal (GI) conditions should be considered when assessing the in vitro digestion of proteins. The research described here compares the extent of hydrolysis of proteins from peanuts and wheat bread, in particular the vicilin-like 7S globulin (Ara h 1) and gliadin, respectively, with three GI scenarios simulating either infant, early phase adult (fed state) or late phase adult (fasted state) conditions. The digestibility of these proteins, in isolation or when naturally present in the respective food matrix, has been evaluated with SDS-PAGE, LC-MS/MS and a spectrophotometric assay. Results from the food matrices showed lower extent of total protein GI digestion under simulated infant conditions, intermediate behaviour under fed state adult conditions and larger extent under fasted state adult conditions. This was also the case for isol...Continue Reading

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Citations

Jan 22, 2021·EFSA Journal·UNKNOWN EFSA Panel on Genetically Modified Organisms (GMO)Antonio Fernandez Dumont
Jun 6, 2021·Food Chemistry·Ousmane SuwarehFrançoise Nau

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Methods Mentioned

BETA
electrophoresis
electrophoretic profile
thermal treatment

Software Mentioned

INFOGEST

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