Human Gastrointestinal Juices Intended for Use in In Vitro Digestion Models.

Food Digestion
Ellen K UllebergG E Vegarud

Abstract

The aim of this study was to characterise the individual human gastric and duodenal juices to be used in in vitro model digestion and to examine the storage stability of the enzymes. Gastroduodenal juices were aspirated, and individual variations in enzymatic activities as well as total volumes, pH, bile acids, protein and bilirubin concentrations were recorded. Individual pepsin activity in the gastric juice varied by a factor of 10, while individual total proteolytic activity in the duodenal juice varied by a factor of 5. The duodenal amylase activity varied from 0 to 52.6 U/ml, and the bile acid concentration varied from 0.9 to 4.5 mM. Pooled gastric and duodenal juices from 18 volunteers were characterised according to pepsin activity (26.7 U/ml), total proteolytic activity (14.8 U/ml), lipase activity (951.0 U/ml), amylase activity (26.8 U/ml) and bile acids (4.5 mM). Stability of the main enzymes in two frozen batches of either gastric or duodenal juice was studied for 6 months. Pepsin activity decreased rapidly and adjusting the pH of gastric juice to 4 did not protect the pepsin from degradation. Lipase activity remained stable for 4 months, however decreased rapidly thereafter even after the addition of protease inhibi...Continue Reading

References

Jan 1, 1991·Gastroenterology·D G KellyE P DiMagno
Jul 1, 1990·Pharmaceutical Research·J B DressmanK M Jarvenpaa
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·L Sánchez-ChiangO Ponce
Apr 1, 1997·Pharmaceutical Research·A LindahlH Lennernäs
Oct 1, 1996·Nature Biotechnology·J D AstwoodR L Fuchs
Dec 23, 1999·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·A LydonG Shorten
Jul 29, 2006·The Journal of Pharmacy and Pharmacology·Mariangeles Perez de la Cruz MorenoPatrick Augustijns
Aug 31, 2006·Alimentary Pharmacology & Therapeutics·N B Roberts
Jan 6, 2007·Digestive Diseases and Sciences·David C Whitcomb, Mark E Lowe
Jul 17, 2007·Gastroenterology·Jonathan GassChaitan Khosla
Aug 28, 2007·Journal of Chromatography. a·Christian E H SchmelzerKlaus Raith
Apr 12, 2008·Pharmaceutical Research·Ekarat JantratidJennifer B Dressman
Jun 26, 2008·Journal of Food Science·F Kong, R P Singh
Jul 16, 2009·Molecular Nutrition & Food Research·Martin WickhamClare Mills
Mar 24, 2010·The British Journal of Nutrition·Ellen K EriksenGerd E Vegarud
Apr 2, 2010·Journal of Agricultural and Food Chemistry·Elizabeth EscuderoFidel Toldrá

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Citations

Jan 23, 2016·Journal of Food Science·Gail M BornhorstSerafim Bakalis
Oct 17, 2015·Food Chemistry·Justyna BorawskaPiotr Minkiewicz
Sep 3, 2013·Food Chemistry·Vera KristinovaTurid Rustad
Jul 26, 2016·Frontiers in Pharmacology·Eleonore FröhlichSharareh Salar-Behzadi
Jan 27, 2017·Annual Review of Food Science and Technology·Gail M Bornhorst
Jun 28, 2017·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Lauren K MarkellBryan Delaney
Jun 28, 2017·The British Journal of Nutrition·Joana Corte-RealTorsten Bohn
Oct 20, 2018·Journal of Food Science·Ernesta MalinauskytėGerd E Vegarud
Feb 7, 2015·Asian-Australasian Journal of Animal Sciences·Mohammad Ashiqul IslamGerd Elisabeth Vegarud
Jan 21, 2020·Critical Reviews in Food Science and Nutrition·Geeshani SomaratneJaspreet Singh
Oct 22, 2019·Current Drug Metabolism·Askhi M Valodara, Kaid Johar Sr
May 17, 2019·Food Science and Biotechnology·Krzysztof DziedzicJarosław Walkowiak
Oct 27, 2020·Nanotechnology·Anna LesiakArtur Podhorodecki
Feb 2, 2020·Plant Science : an International Journal of Experimental Plant Biology·Juliana CotabarrenWalter David Obregón
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·M AlmingerC N Santos
Mar 23, 2021·Critical Reviews in Food Science and Nutrition·Yun-Shan WeiHong Wu
Jul 1, 2021·Expert Opinion on Drug Discovery·Xianyin LaiMohamed E H ElSayed
Aug 16, 2017·Journal of Agricultural and Food Chemistry·Isabelle F YangBhimanagouda S Patil

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Methods Mentioned

BETA
protein assay

Software Mentioned

SAS

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