Abstract
The aim of this study was the characterization of siloran-derived composite eluates in conjunction with their putative impact on human gingival keratinocytes (HGK), i.e. levels of total RNA and induction of apoptosis compared to a methacrylate-based material. Standardized Filtek™ Silorane specimens (n = 20) were subjected to scanning ion monitoring to detect monomer masses between 100 and 1000, after storage in human saliva, and 75% ethanol for up to 28 days. In order to evaluate the effect on cells, HGK were exposed to eluates from Filtek™ Silorane, Filtek™ Supreme XT and control medium for 1 and 4 days, prior to isolation of total RNA, and Annexin-5 fluorescence labeling indicating induction of apoptosis. Irrespective of the mode and storage time, SIM identified discrete peaks, corresponding to masses of "393" and "337". In response to both composite eluates, an effect on HGK was reflected by drastically reduced levels of isolated total RNA at each time period (after 1 day: control: 302 ng/μl; Filtek™ Silorane: 128 ng/μl, Filtek™ Supreme XT: 129 ng/μl and after 4 days: control: 528 ng/μl; Filtek™ Silorane: 162 ng/μl, Filtek™ Supreme XT: 166 ng/μl). Exposure to eluates from both composite materials yielded apoptosis induction ...Continue Reading
References
Mar 1, 1990·Journal of Dental Research·K H Chung
Nov 1, 1995·Dental Materials : Official Publication of the Academy of Dental Materials·S Y LeeD L Menis
May 19, 1998·European Journal of Oral Sciences·W Geurtsen
Jul 11, 1998·Journal of Biomedical Materials Research·W GeurtsenG Leyhausen
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Dec 11, 2002·Journal of Oral Rehabilitation·T-H YoonC-W Kim
Dec 24, 2003·Journal of Dental Research·H SchweiklW Weinmann
Feb 1, 2005·Dental Materials : Official Publication of the Academy of Dental Materials·Wolfgang WeinmannRainer Guggenberger
May 13, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Irini D Sideridou, Dimitris S Achilias
Jan 24, 2006·Acta Odontologica Scandinavica·Alexander FranzAndreas Schedle
Feb 14, 2006·Archives of Toxicology·Franz-Xaver ReichlReinhard Hickel
Oct 6, 2006·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M Goël BrackettJ C Wataha
Oct 20, 2006·Dental Materials : Official Publication of the Academy of Dental Materials·Joachim VolkWerner Geurtsen
Dec 21, 2006·Differentiation; Research in Biological Diversity·Mariana Roesch-ElyPascal Tomakidi
Apr 6, 2007·Dental Materials : Official Publication of the Academy of Dental Materials·Olga PolydorouKlaus Kümmerer
Sep 9, 2008·Dental Materials : Official Publication of the Academy of Dental Materials·Olga PolydorouKlaus Kümmerer
Feb 7, 2009·European Journal of Oral Sciences·Olga PolydorouKlaus Kümmerer
Apr 21, 2009·Dental Materials : Official Publication of the Academy of Dental Materials·Ana R BenettiJacques Devaux
May 5, 2009·Dental Materials : Official Publication of the Academy of Dental Materials·Olga PolydorouKlaus Kümmerer
May 12, 2009·Chemico-biological Interactions·Tomasz PoplawskiJanusz Blasiak
Jul 3, 2009·Dental Materials : Official Publication of the Academy of Dental Materials·Chiara O NavarraLorenzo Breschi
Feb 17, 2010·European Journal of Oral Sciences·Hilde M KopperudInger S Kleven
Jan 19, 2011·Clinical Oral Investigations·Stephanie KrifkaHelmut Schweikl
Sep 29, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Olga PolydorouKlaus Kümmerer
Citations
May 3, 2013·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Ghania Nina AttikBrigitte Grosgogeat
Sep 17, 2013·PloS One·Ali Al-AhmadKaren Lienkamp
Sep 9, 2015·Dental Materials : Official Publication of the Academy of Dental Materials·Simon Daniel SchulzOlga Polydorou
Feb 9, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·K SiemerO Polydorou