Human gingival keratinocytes express E-selectin (CD62E)

Oral Diseases
E R PietrzakL J Walsh

Abstract

E-selectin (CD62E) is an adhesion molecule that participates in the binding of leukocytes to activated blood vascular endothelium. The present study was undertaken to characterise the pattern of E-selectin expression on epithelial cells of the gingival crevice and oral epithelium. A panel of six anti-E-selectin monoclonal antibodies was reacted with cryosections of human gingiva and cytospins of cultured gingival keratinocytes. Three antibodies raised against leukocyte binding epitopes of the molecule (H4/18, H18/7, 1.2B6) were reactive with gingival keratinocytes, three were negative (4D10, BBA1, BBA2), while all six reacted with endothelial cells. Staining with H18/7 and 1.2B6 was weaker and more variable than with H4/18. In cell culture, levels of E-selectin expression reduced slowly, and were only modestly increased by treatment with exogenous TNF alpha, a known inducer of E-selectin on endothelium. E-selectin epitopes are expressed on gingival keratinocytes. These binding epitopes may be involved in the traffic of leukocytes in this tissue compartment.

References

Jun 1, 1992·Journal of Cutaneous Pathology·L J Walsh, G F Murphy
Mar 1, 1992·Immunology Today·Y ShimizuS Shaw
Feb 28, 1991·Nature·L J PickerE C Butcher
May 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J WalshG F Murphy
Oct 1, 1990·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·L J WalshG J Seymour
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M P BevilacquaM A Gimbrone
Jan 1, 1988·The Journal of Investigative Dermatology·L J Walsh, G J Seymour
Jan 1, 1994·Journal of Periodontal Research·E GemmellG J Seymour
Jul 1, 1995·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·L J WalshN W Savage
Nov 1, 1995·Journal of Periodontal Research·E R PietrzakL J Walsh
Jul 1, 1996·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·E R PietrzakL J Walsh

❮ Previous
Next ❯

Citations

Apr 12, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A I Rojas, A R Ahmed

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.