PMID: 49057Jun 1, 1975Paper

Human globin gene analysis for a patient with beta-o/delta beta-thalassemia

Proceedings of the National Academy of Sciences of the United States of America
S OttolenghiC S Pitcher

Abstract

Complementary DNA (cDNA) was prepared with RNA-dependent DNA polymerase from human globin messenger RNA (mRNA). Annealing and translation experimenta with total mRNA from circulating cells from a patient with heterozygous beta/heterozygous beta-delta-o thalassemia (beta-o/delta beta-o-thalassemia) demonstrated no detectable mRNA for beta-globin. cDNA enriched in sequences homologous to beta-globin mRNA was prepared by hydroxylapatite fractionation of hybrids formed between beta-o/delta beta-o-thalassemic mRNA and cDNA made from mRNA from a patient with alpha-thalassemia (hemoglobin H disease). The rate of annealing of this beta-enriched cDNA to normal human nuclear DNA was that of a sequence present as only a single copy per haploid genome. The beta-enriched cDNA annealed to the beta-o-delta beta-o-thalassemia total DNA with approximately the same kinetics as to normal DNA, indicating that no total gene deletion of beta-globin genes from the diploid genome has occurred, although the accuracy of the technique could not exclude with certainty a partial deletion or a deletion of a beta-globin gene from only one of the haploid genomes. This demonstrates that at least one of the beta-o- or the delta beta-o-thalassemia haploid genome...Continue Reading

References

Jan 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·W G LanyonR Williamson
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D HousmanE J Benz
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·D L KacianA Bank
Feb 1, 1974·Nature·K S Gummerson, R Williamson
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Jul 19, 1972·Nature: New Biology·F ConconiS Volpato
Jan 1, 1974·Cold Spring Harbor Symposia on Quantitative Biology·J O Bishop, K B Freeman
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B E Roberts, B M Paterson
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·R GambinoA Bank
Dec 1, 1972·Archives of Biochemistry and Biophysics·T H HuismanA G Kendall
Mar 14, 1972·Biochimica Et Biophysica Acta·R P PerryJ R Greenberg
Apr 14, 1972·Biochemical and Biophysical Research Communications·A V RobertsJ B Clegg
Aug 1, 1972·Analytical Biochemistry·A HellJ Paul
Dec 1, 1971·The Journal of Clinical Investigation·E J Benz, B G Forget
Oct 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·D G NathanD Housman
Mar 1, 1969·British Journal of Haematology·D J WeatherallP Wasi
Dec 11, 1965·Nature·D J WeatherallM A Naughton

❮ Previous
Next ❯

Citations

Sep 20, 1978·Molecular & General Genetics : MGG·P HumphriesJ Paul
Aug 1, 1978·Developmental Biology·J F JacksonD I de Pomerai
Aug 18, 2000·Free Radical Biology & Medicine·E A Meagher, G A FitzGerald
Nov 18, 1976·The New England Journal of Medicine·Y W KanA M Dozy
Dec 22, 1977·The New England Journal of Medicine·A W Nienhuis, E J Benz
Dec 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·Y W KanH E Varmus
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A W NienhuisE J Benz
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·N J Proudfoot, F E Baralle
Jun 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J C Chang, Y W Kan
Sep 11, 1981·Nucleic Acids Research·N MoschonasR A Flavell
Apr 10, 2002·American Journal of Therapeutics·W Stewart Hillis
Mar 1, 1978·British Journal of Haematology·D J WeatherallM J Lewis
Feb 1, 1976·Journal of Medical Genetics·D J WeatherallG R Serjeant
Jan 6, 1999·Proceedings of the National Academy of Sciences of the United States of America·B F McAdamG A FitzGerald
Jun 2, 1976·Biochimica Et Biophysica Acta·R DalgleishP Tolstoshev
Jan 1, 1981·Critical Reviews in Clinical Laboratory Sciences·D Pitcher
Jan 1, 1980·Annals of the New York Academy of Sciences·P WasiS Fucharoen
Jan 1, 1980·Annals of the New York Academy of Sciences·F ConconiC M Perrotta
Jan 1, 1980·Annals of the New York Academy of Sciences·A BankS Spence
May 1, 1980·British Journal of Haematology·D M HuntG W Marsh
Jan 12, 1976·Biochemical and Biophysical Research Communications·R WilliamsonT McShane
Aug 17, 2016·Journal of Clinical Laboratory Analysis·Liang-Yin KeTa-Chih Liu
Jan 1, 1978·CRC Critical Reviews in Biochemistry·A Bank, F Ramirez
Aug 20, 2002·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·T G de AndradeF F Costa
Apr 1, 1996·Stroke; a Journal of Cerebral Circulation·C Patrono, G J Roth
Dec 31, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·C Patrono, G A FitzGerald
Aug 10, 1978·Nature·A M GianniS Ottolenghi
Dec 1, 1979·European Journal of Biochemistry·A ViottiC Soave
Aug 15, 1977·European Journal of Biochemistry·P TolstoshevM Benabadji
Oct 3, 1977·European Journal of Biochemistry·P ComiG Masera

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anemia

Anemia develops when your blood lacks enough healthy red blood cells. Anemia of inflammation (AI, also called anemia of chronic disease) is a common, typically normocytic, normochromic anemia that is caused by an underlying inflammatory disease. Here is the latest research on anemia.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Y W KanH E Varmus
British Journal of Haematology
J PritchardD J Weatherall
Proceedings of the National Academy of Sciences of the United States of America
G F TempleY W Kan
© 2021 Meta ULC. All rights reserved