Human glomerular epithelial cells synthesize and secrete the third component of complement

Nephron
A MoutabarrikT Seya

Abstract

Complement proteins in serum are mainly synthesized by hepatocytes. Recently, many cell types have been reported to synthesize complement in various tissues. In this study, we report the synthesis and secretion of the third component of complement (C3) by cultured glomerular epithelial cells (GEC). Using reverse transcriptase polymerase reaction, we have found that GEC and whole kidney expressed the C3 mRNA for C3. By ELISA, we have found that C3 was secreted in culture supernatants harvested from cultured GEC. The secretion of C3 is regulated by proinflammatory cytokines (IL-1 beta, TNF-alpha and IL-6). IL-1 beta is shown to be the most potent stimulator of C3 secretion from GEC. The exact significance of C3 produced at glomerular site is not clear, but its upregulation by proinflammatory cytokines may suspect a role in local activation of complement which may lead to glomerular injury. We further studied the expression of C3 step regulatory proteins (membrane cofactor protein (MCP), decay-accelerating factor (DAF), CR-1 and CD59 (a terminal step regulatory protein) by cultured GEC. Treatment of GEC by proinflammatory cytokines IL-1 beta, TGF-beta, TNF-alpha and IL-6 did not modify the expression of MCP, DAF and CR-1 whereas a...Continue Reading

Citations

Dec 9, 1998·Kidney International·M Nangaku
May 7, 1999·Kidney International·M Kitamura, L G Fine
Feb 11, 2009·Molecular Reproduction and Development·Wang-Ngai ChowWilliam Shu-Biu Yeung
Dec 12, 2001·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·M J BownR D Sayers
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H SekineG S Gilkeson
Feb 21, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Naobumi HosoganeTatsuo Suda
Nov 25, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·A ChenW H Lee

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