PMID: 8589523Aug 1, 1995Paper

Human glucokinase regulatory protein (GCKR): cDNA and genomic cloning, complete primary structure, and chromosomal localization

Mammalian Genome : Official Journal of the International Mammalian Genome Society
J P WarnerD T Bonthron

Abstract

Null mutations in the glucokinase (GCK) gene can cause autosomal dominant type 2 diabetes (maturity onset diabetes of the young, MODY); however, MODY is genetically heterogeneous. In both liver and pancreatic islet, glucokinase is subject to inhibition by a regulatory protein (GCKR). Given the role of GCK in MODY, GCKR is itself a candidate type 2 diabetes susceptibility gene. Here we describe the structure of full-length (2.2 kb) cDNA for human GCKR, from the hepatoblastoma cell line HepG2. The human GCKR translation product has 625 amino acids and a predicted molecular weight of 68,700. It has 88% amino acid identity to rat GCKR. Yeast artificial chromosomes (YAC clones) containing human GCKR were isolated, and the gene was mapped to Chromosome (Chr) 2p23 by fluorescent in situ hybridization and somatic cell hybrid analysis.

References

Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G I BellN J Cox
Jul 5, 1990·European Journal of Biochemistry·W J MalaisseE Van Schaftingen
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D PinkelJ W Gray
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
Mar 11, 1993·The New England Journal of Medicine·P FroguelP Passa

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Citations

Jun 1, 1996·Mammalian Genome : Official Journal of the International Mammalian Genome Society·B E HaywardD T Bonthron
Oct 23, 2009·Journal of Molecular Neuroscience : MN·Luca JáromiBéla Melegh
Mar 1, 2002·Diabetic Medicine : a Journal of the British Diabetic Association·J BentzenM Fenger
Dec 10, 1999·Proceedings of the National Academy of Sciences of the United States of America·D FarrellyN Hariharan
Jan 13, 2006·Diabetic Medicine : a Journal of the British Diabetic Association·B KøsterJ Bentzen
Aug 25, 2016·Journal of Clinical Laboratory Analysis·Mauren Isfer Anghebem-OliveiraFabiane Gomes de Moraes Rego
Jan 6, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jun-Seop ShinMasakazu Shiota
Jan 18, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mohammed H MukhtarLoranne Agius
Jul 25, 2008·The Biochemical Journal·Loranne Agius

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