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Human granulocyte 6 phosphogluconate dehydrogenase. Purification by elective elution with NADP+, immunological and kinetic properties

Biochimie

Jan 1, 1975

D CottreauJean-Pierre Marie

PMID: 238666

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Abstract

Human granulocyte 6 phosphogluconate dehydrogenase has been totally purified from a single patient with chronic granulocytic leukaemia. 48 mg of protein, of specific activity 20 IU per mg of protein, have been obtained in the course of three different steps only. The overall yield was 3...read more

Mentioned in this Paper

Sulfhydryl Reagents
Inactivation
Leukemia, Monocytic, Chronic
Ion-Exchange Chromatography Procedure
Slow-K
NADP
Striadyne
Sample Fixation
Metabolic Inhibition
Granulocyte
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  • Human granulocyte 6 phosphogluconate dehydrogenase. Purification by elective elution with NADP+, immunological and kinetic properties

    Biochimie

    Jan 1, 1975

    D CottreauJean-Pierre Marie

    PMID: 238666

    DOI:

    Abstract

    Human granulocyte 6 phosphogluconate dehydrogenase has been totally purified from a single patient with chronic granulocytic leukaemia. 48 mg of protein, of specific activity 20 IU per mg of protein, have been obtained in the course of three different steps only. The overall yield was 3...read more

    Mentioned in this Paper

    Sulfhydryl Reagents
    Inactivation
    Leukemia, Monocytic, Chronic
    Ion-Exchange Chromatography Procedure
    Slow-K

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