PMID: 9440551Jan 24, 1998Paper

Human granulocyte-macrophage colony-stimulating factor (hGM-CSF)-dependent in vitro and in vivo proliferation and differentiation of all hematopoietic progenitor cells in hGM-CSF receptor transgenic mice

The Journal of Allergy and Clinical Immunology
I NishijimaK Arai

Abstract

To examine the relation between receptor expression and differentiation of hematopoietic cells, we produced transgenic mice that constitutively expressed the human granulocyte-macrophage colony stimulating factor (hGM-CSF) receptor at almost all stages of hematopoietic cell development. The high-affinity GM-CSF receptor is species specific, allowing analysis of the specific effects of hGM-CSF in our mouse model. Proliferation and differentiation of hematopoietic progenitor cells from transgenic mice were analyzed by means of methylcellulose colony-forming assay and in vivo treatment with hGM-CSF, respectively. We found that hGM-CSF supported various types of colonies, including granulocyte-macrophage, mast cell, megakaryocyte, blast cell, and mixed hematopoietic colonies, whereas mouse GM-CSF supported only granulocyte-macrophage colonies. In addition, hGM-CSF generated erythrocyte colonies in the absence of erythropoietin. Furthermore, in vivo administration of hGM-CSF to transgenic mice resulted in a dose-dependent increase in reticulocytes and white blood cells in the peripheral blood. The spleens of the mice showed gross enlargement, mainly caused by an increase of erythroid cells and their progenitors. Taken together, thes...Continue Reading

Citations

Nov 30, 2002·Seminars in Immunology·Ellen V Rothenberg
Mar 29, 2013·Cytokine & Growth Factor Reviews·Timothy R HercusAngel F Lopez
Apr 30, 2015·PloS One·Renuka SubramaniamHomayoun Shams
May 9, 2001·International Reviews of Immunology·T Nagamura-InoueK Ozato
Oct 8, 2003·Experimental Biology and Medicine·Asim B Abdel-MageedKrishna C Agrawal

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