Human granulosa luteal cell oxidative phosphorylation function is not affected by age or ovarian response

Fertility and Sterility
Yoel ShufaroAnn Saada

Abstract

To safely prepare a functional autologous mitochondrial concentrate (MC) from follicular fluid (FF) cells, and to determine the effect of age and ovarian response on the oxidative phosphorylation (OXPHOS). The nontoxicity of the MC was confirmed in human and mouse oocytes. The OXPHOS function was assessed by measuring the activity of succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), and adenosine triphosphate (ATP) production in comparison with citrate synthase. The integrity of the mitochondrial DNA (mtDNA) was demonstrated by polymerase chain reaction (PCR). Tertiary hospital. A total of 40 patients undergoing IVF of heterogeneous ages and ovarian response. Superovulated 8- to 12-week-old female B(6)C(3)F(1) mice. None. A system for the preparation of functional nontoxic MC was established. The correlation between the mitochondrial mass and function to age and ovarian response was calculated. The integrity of mtDNA was demonstrated. After injection into mouse oocytes, the MC did not interfere with parthenogenetic development. The MC OXPHOS function was intact. Total activity of SDH and COX was in correlation with the retrieved oocytes number, and in reverse correlation with age. However, after correction to the mi...Continue Reading

References

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Citations

Feb 11, 2015·Developmental Cell·Miri Stolovich-RainYuval Dor
Aug 27, 2016·Human Reproduction Update·Pascale May-PanloupPascal Reynier
Oct 19, 2017·Human Reproduction·Yifan LiuYuanqing Yao
Oct 24, 2019·Journal of Alzheimer's Disease : JAD·Keren NitzanHanna Rosenmann
Aug 22, 2020·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Gustavo N CecchinoJuan A García-Velasco
Dec 18, 2013·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·T Strowitzki
Jan 19, 2021·Molecular Genetics and Metabolism Reports·Tova HershkovitzHagit Baris Feldman

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